Reactions of isolated mono-molecular protein films

Adam W. Perriman, Duncan J. Mcgillivray, John W. White

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    Wash-out of the forming solution from mono-molecular casein films at the air-water interface is demonstrated and allows the perfusion of the subphase by inorganic and protein solutions. In this way, using X-ray reflectometry, we have observed the effects of calcium binding to β-casein films and the inter-protein association of β-casein and κ-casein in an order-specific manner. The method is of general utility for monolayer reactions and here the relevance to self-assembly of the casein micelle is discussed.

    Original languageEnglish
    Pages (from-to)2192-2198
    Number of pages7
    JournalSoft Matter
    Volume4
    Issue number11
    DOIs
    Publication statusPublished - 2008

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