Real-time monitoring of amyloid growth in a rigid gel matrix

Roshan C. Dalpadado, Hendrik Maat, John A. Carver, Damien Hall*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    We demonstrate the real-time monitoring of the growth of amyloid-protein aggregates in a semi-rigid gel environment constructed from a 5% w/v gelatin solution. The kinetics of amyloid fibril growth from reduced and carboxy-methylated κ-casein occurring in the gel medium was contrasted against that obtained in a regular solution assay. Aggregation kinetics were recorded using Thioflavin T fluorescence. Transmission electron microscopy was used to confirm the aggregates' existence and morphology. The current demonstration of controlled amyloid growth in a gel environment represents the first step towards development of an experimental model for investigating the role of spatial and medium factors in the kinetics of aggregation-based proteopathies.

    Original languageEnglish
    Pages (from-to)13-16
    Number of pages4
    JournalAnalytical Biochemistry
    Volume511
    DOIs
    Publication statusPublished - 15 Oct 2016

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