Reconstruction of 3D ion beam micro-tomography data for applications in Cell Biology

C. Habchi; D. T. Nguyen; Ph Barberet; S. Incerti; Ph Moretto; A. Sakellariou; H. Seznec

doi:10.1016/j.nimb.2009.03.070

Reconstruction of 3D ion beam micro-tomography data for applications in Cell Biology

C. Habchi^*, D. T. Nguyen, Ph Barberet, S. Incerti, Ph Moretto, A. Sakellariou, H. Seznec

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The DISRA (Discrete Image Space Reconstruction Algorithm) reconstruction code, created by A. Sakellariou, was conceived for the ideal case of complete three-dimensional (3D) PIXET (Particle Induced X-ray Emission Tomography) data. This implies two major difficulties for biological samples: first, the long duration of such experiments and second, the subsequent damage that occurs on such fragile specimens. For this reason, the DISRA code was extended at CENBG in order to probe isolated PIXET slices, taking into account the sample structure and mass density provided by 3D STIMT (Scanning Transmission Ion Microscopy Tomography) in the volume of interest. This modified version was tested on a phantom sample and first results on human cancer cells are also presented.

Original language	English
Pages (from-to)	2107-2112
Number of pages	6
Journal	Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume	267
Issue number	12-13
DOIs	https://doi.org/10.1016/j.nimb.2009.03.070
Publication status	Published - 15 Jun 2009

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title = "Reconstruction of 3D ion beam micro-tomography data for applications in Cell Biology",

abstract = "The DISRA (Discrete Image Space Reconstruction Algorithm) reconstruction code, created by A. Sakellariou, was conceived for the ideal case of complete three-dimensional (3D) PIXET (Particle Induced X-ray Emission Tomography) data. This implies two major difficulties for biological samples: first, the long duration of such experiments and second, the subsequent damage that occurs on such fragile specimens. For this reason, the DISRA code was extended at CENBG in order to probe isolated PIXET slices, taking into account the sample structure and mass density provided by 3D STIMT (Scanning Transmission Ion Microscopy Tomography) in the volume of interest. This modified version was tested on a phantom sample and first results on human cancer cells are also presented.",

keywords = "3D imaging, Computed tomography, Human cell, Micro-tomography, Non-destructive characterisation, Nuclear microprobe, Particle Induced X-ray Emission, Scanning Transmission Ion Microscopy",

author = "C. Habchi and Nguyen, {D. T.} and Ph Barberet and S. Incerti and Ph Moretto and A. Sakellariou and H. Seznec",

year = "2009",

month = jun,

day = "15",

doi = "10.1016/j.nimb.2009.03.070",

language = "English",

volume = "267",

pages = "2107--2112",

journal = "Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms",

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TY - JOUR

T1 - Reconstruction of 3D ion beam micro-tomography data for applications in Cell Biology

AU - Habchi, C.

AU - Nguyen, D. T.

AU - Barberet, Ph

AU - Incerti, S.

AU - Moretto, Ph

AU - Sakellariou, A.

AU - Seznec, H.

PY - 2009/6/15

Y1 - 2009/6/15

N2 - The DISRA (Discrete Image Space Reconstruction Algorithm) reconstruction code, created by A. Sakellariou, was conceived for the ideal case of complete three-dimensional (3D) PIXET (Particle Induced X-ray Emission Tomography) data. This implies two major difficulties for biological samples: first, the long duration of such experiments and second, the subsequent damage that occurs on such fragile specimens. For this reason, the DISRA code was extended at CENBG in order to probe isolated PIXET slices, taking into account the sample structure and mass density provided by 3D STIMT (Scanning Transmission Ion Microscopy Tomography) in the volume of interest. This modified version was tested on a phantom sample and first results on human cancer cells are also presented.

AB - The DISRA (Discrete Image Space Reconstruction Algorithm) reconstruction code, created by A. Sakellariou, was conceived for the ideal case of complete three-dimensional (3D) PIXET (Particle Induced X-ray Emission Tomography) data. This implies two major difficulties for biological samples: first, the long duration of such experiments and second, the subsequent damage that occurs on such fragile specimens. For this reason, the DISRA code was extended at CENBG in order to probe isolated PIXET slices, taking into account the sample structure and mass density provided by 3D STIMT (Scanning Transmission Ion Microscopy Tomography) in the volume of interest. This modified version was tested on a phantom sample and first results on human cancer cells are also presented.

KW - 3D imaging

KW - Computed tomography

KW - Human cell

KW - Micro-tomography

KW - Non-destructive characterisation

KW - Nuclear microprobe

KW - Particle Induced X-ray Emission

KW - Scanning Transmission Ion Microscopy

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U2 - 10.1016/j.nimb.2009.03.070

DO - 10.1016/j.nimb.2009.03.070

M3 - Article

SN - 0168-583X

VL - 267

SP - 2107

EP - 2112

JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

IS - 12-13

ER -

Reconstruction of 3D ion beam micro-tomography data for applications in Cell Biology

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