Relative abundance of Mycobacterium in ovine Johne's disease

Andy Leu; Paul Pavli; David Gordon; Jeff Cave; Jacek M Gowzdz; Nick Linden; Grant Rawlin; Gwen Allison; Gwen Allison; Claire O'Brien; Claire O'Brien

doi:10.1071/MA15010

Relative abundance of Mycobacterium in ovine Johne's disease

Andy Leu, Paul Pavli, David Gordon, Jeff Cave, Jacek M Gowzdz, Nick Linden, Grant Rawlin, Gwen Allison, Gwen Allison, Claire O'Brien, Claire O'Brien

Research output: Contribution to journal › Article › peer-review

Abstract

No study has determined what proportion of the total microbiota comprises the genus Mycobacterium in ovine Johnes disease (OJD) tissues. We aimed to assess the relative abundance of Mycobacterium in the ileocaecal lymph node, involved and uninvolved ileal mucosa from sheep with and without OJD, using three extraction methods. Eight sheep, four with and four without OJD, were recruited. Pyrosequencing of the 16Sr RNA gene amplicons for all samples revealed that Mycobacterium represented between 0-92% (average 38%) of the total microbiota of samples from sheep with OJD, and 0-85% (average 13%) of sheep without OJD. Only sheep with OJD had samples that were positive for the IS900 (MAP) element. Mycobacterial strains other than MAP may provide competitive exclusion of MAP and should be further investigated.

Original language	English
Pages (from-to)	32-36
Journal	Microbiology Australia
Volume	36
Issue number	1
DOIs	https://doi.org/10.1071/MA15010
Publication status	Published - 2015

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title = "Relative abundance of Mycobacterium in ovine Johne's disease",

abstract = "No study has determined what proportion of the total microbiota comprises the genus Mycobacterium in ovine Johnes disease (OJD) tissues. We aimed to assess the relative abundance of Mycobacterium in the ileocaecal lymph node, involved and uninvolved ileal mucosa from sheep with and without OJD, using three extraction methods. Eight sheep, four with and four without OJD, were recruited. Pyrosequencing of the 16Sr RNA gene amplicons for all samples revealed that Mycobacterium represented between 0-92% (average 38%) of the total microbiota of samples from sheep with OJD, and 0-85% (average 13%) of sheep without OJD. Only sheep with OJD had samples that were positive for the IS900 (MAP) element. Mycobacterial strains other than MAP may provide competitive exclusion of MAP and should be further investigated.",

author = "Andy Leu and Paul Pavli and David Gordon and Jeff Cave and Gowzdz, {Jacek M} and Nick Linden and Grant Rawlin and Gwen Allison and Gwen Allison and Claire O'Brien and Claire O'Brien",

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AU - Leu, Andy

AU - Pavli, Paul

AU - Gordon, David

AU - Cave, Jeff

AU - Gowzdz, Jacek M

AU - Linden, Nick

AU - Rawlin, Grant

AU - Allison, Gwen

AU - O'Brien, Claire

PY - 2015

Y1 - 2015

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AB - No study has determined what proportion of the total microbiota comprises the genus Mycobacterium in ovine Johnes disease (OJD) tissues. We aimed to assess the relative abundance of Mycobacterium in the ileocaecal lymph node, involved and uninvolved ileal mucosa from sheep with and without OJD, using three extraction methods. Eight sheep, four with and four without OJD, were recruited. Pyrosequencing of the 16Sr RNA gene amplicons for all samples revealed that Mycobacterium represented between 0-92% (average 38%) of the total microbiota of samples from sheep with OJD, and 0-85% (average 13%) of sheep without OJD. Only sheep with OJD had samples that were positive for the IS900 (MAP) element. Mycobacterial strains other than MAP may provide competitive exclusion of MAP and should be further investigated.

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JO - Microbiology Australia

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Relative abundance of Mycobacterium in ovine Johne's disease

Abstract

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