Scopolamine content of a Duboisia hybrid in callus cultures: Utility of Germline, Somatic and ctDNA Testing in Adults With Cancer

Scopolamine levels in a Duboisia leichhardtii × D. myoporoides hybrid were determined by radioimmunoassay and gas liquid chromatography in explants, callus and at various stages of plant regeneration. Scopolamine could not be detected in undifferentiated callus or differentiated shoots. Scopolamine only occurred in cultures which had developed roots. After 16 weeks, regenerated plants contained scopolamine at a level comparable with that in seedlings of a similar age. The scopolamine content of regenerated field-grown plants had scopolamine levels similar to conventionally vegetatively-propagated hybrid plants, except that littorine was the second major alkaloid, rather than hyoscyamine.