Selection of a peptide ligand to the p75 neurotrophin receptor death domain and determination of its binding sites by NMR

Leopold L. Ilag; Christine Rottenberger; Edvards Liepinsh; Günter Wellnhofer; Fritz Rudert; Gottfried Otting; Leodevico L. Ilag

doi:10.1006/bbrc.1999.0101

Selection of a peptide ligand to the p75 neurotrophin receptor death domain and determination of its binding sites by NMR

Leopold L. Ilag, Christine Rottenberger, Edvards Liepinsh, Günter Wellnhofer, Fritz Rudert, Gottfried Otting^*, Leodevico L. Ilag

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The p75 neurotrophin receptor (p75(NTR)) contains a conserved death domain module similar to that of the cytotoxic receptors Fas and TNFR-1. Here, we describe the selection of peptide ligands from a combinatorial library using a variation of the selectively-infective phage (SIP) method directed to the death domain of p75(NTR). The binding sites on the death domain of p75(NTR) were identified for a 15 amino acid residue peptide by nuclear magnetic resonance (NMR) spectroscopy. The selected peptides may be useful for probing the function of the p75(NTR) death domain and aid in defining its downstream signalling mechanism.

Original language	English
Pages (from-to)	104-109
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	255
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1999.0101
Publication status	Published - 5 Feb 1999
Externally published	Yes

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10.1006/bbrc.1999.0101

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title = "Selection of a peptide ligand to the p75 neurotrophin receptor death domain and determination of its binding sites by NMR",

abstract = "The p75 neurotrophin receptor (p75(NTR)) contains a conserved death domain module similar to that of the cytotoxic receptors Fas and TNFR-1. Here, we describe the selection of peptide ligands from a combinatorial library using a variation of the selectively-infective phage (SIP) method directed to the death domain of p75(NTR). The binding sites on the death domain of p75(NTR) were identified for a 15 amino acid residue peptide by nuclear magnetic resonance (NMR) spectroscopy. The selected peptides may be useful for probing the function of the p75(NTR) death domain and aid in defining its downstream signalling mechanism.",

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AU - Ilag, Leopold L.

AU - Rottenberger, Christine

AU - Liepinsh, Edvards

AU - Wellnhofer, Günter

AU - Rudert, Fritz

AU - Otting, Gottfried

AU - Ilag, Leodevico L.

PY - 1999/2/5

Y1 - 1999/2/5

N2 - The p75 neurotrophin receptor (p75(NTR)) contains a conserved death domain module similar to that of the cytotoxic receptors Fas and TNFR-1. Here, we describe the selection of peptide ligands from a combinatorial library using a variation of the selectively-infective phage (SIP) method directed to the death domain of p75(NTR). The binding sites on the death domain of p75(NTR) were identified for a 15 amino acid residue peptide by nuclear magnetic resonance (NMR) spectroscopy. The selected peptides may be useful for probing the function of the p75(NTR) death domain and aid in defining its downstream signalling mechanism.

AB - The p75 neurotrophin receptor (p75(NTR)) contains a conserved death domain module similar to that of the cytotoxic receptors Fas and TNFR-1. Here, we describe the selection of peptide ligands from a combinatorial library using a variation of the selectively-infective phage (SIP) method directed to the death domain of p75(NTR). The binding sites on the death domain of p75(NTR) were identified for a 15 amino acid residue peptide by nuclear magnetic resonance (NMR) spectroscopy. The selected peptides may be useful for probing the function of the p75(NTR) death domain and aid in defining its downstream signalling mechanism.

UR - https://www.scopus.com/pages/publications/0033525002

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DO - 10.1006/bbrc.1999.0101

M3 - Article

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VL - 255

SP - 104

EP - 109

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Selection of a peptide ligand to the p75 neurotrophin receptor death domain and determination of its binding sites by NMR

Abstract

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