Sensitivity-enhanced double-TROSY experiment for simultaneous measurement of one-bond15N-1H, 15N-13C' and two-bond1H-13C' couplings

Masaru Hoshino; Gottfried Otting

doi:10.1016/j.jmr.2004.08.020

Sensitivity-enhanced double-TROSY experiment for simultaneous measurement of one-bond¹⁵N-¹H, ¹⁵N-¹³C' and two-bond¹H-¹³C' couplings

Masaru Hoshino, Gottfried Otting^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A recently published experiment for the measurement of ¹ _HN, ¹NC', and ²J_HC' coupling constants [J. Am. Chem. Soc. 125 (2003) 11504] was modified to yield a double-TROSY experiment which selects 1 of the 16 multiplet components from a ¹⁵N-HSQC spectrum recorded of a uniformly ¹⁵N/ ¹³C-labelled protein. Subspectra containing any 1 of the 16 multiplet components can be generated allowing accurate coupling constant measurements. The experiment is sensitivity enhanced, turning all magnetization components precessing during the evolution time into observable magnetization during the detection time. The experiment is discussed with regard to the previously published α/β-filtered HN(α/β-NC'-J) experiment [J. Magn. Reson. 140 (1999), 32] which measures the same coupling constants.

Original language	English
Pages (from-to)	270-276
Number of pages	7
Journal	Journal of Magnetic Resonance
Volume	171
Issue number	2
DOIs	https://doi.org/10.1016/j.jmr.2004.08.020
Publication status	Published - Dec 2004

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10.1016/j.jmr.2004.08.020

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title = "Sensitivity-enhanced double-TROSY experiment for simultaneous measurement of one-bond15N-1H, 15N-13C' and two-bond1H-13C' couplings",

abstract = "A recently published experiment for the measurement of 1 HN, 1NC', and 2JHC' coupling constants [J. Am. Chem. Soc. 125 (2003) 11504] was modified to yield a double-TROSY experiment which selects 1 of the 16 multiplet components from a 15N-HSQC spectrum recorded of a uniformly 15N/ 13C-labelled protein. Subspectra containing any 1 of the 16 multiplet components can be generated allowing accurate coupling constant measurements. The experiment is sensitivity enhanced, turning all magnetization components precessing during the evolution time into observable magnetization during the detection time. The experiment is discussed with regard to the previously published α/β-filtered HN(α/β-NC'-J) experiment [J. Magn. Reson. 140 (1999), 32] which measures the same coupling constants.",

keywords = "Couplings constants, Sensitivity enhancement, Spin-state selective editing, TROSY",

author = "Masaru Hoshino and Gottfried Otting",

year = "2004",

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AU - Otting, Gottfried

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Sensitivity-enhanced double-TROSY experiment for simultaneous measurement of one-bond¹⁵N-¹H, ¹⁵N-¹³C' and two-bond¹H-¹³C' couplings

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