Sensitivity enhancement in (HCA)CONH experiments

Rutger Folmer; Gottfried Otting

doi:10.1023/A:1008308106108

Sensitivity enhancement in (HCA)CONH experiments

Rutger Folmer, Gottfried Otting^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A novel sensitivity-enhancement technique is proposed for experiments which correlate protein backbone resonances and start with magnetization from ¹³C(α)-¹H(α) groups. The technique is based on replenishing magnetization lost by dipole-CSA cross-correlated relaxation of the ¹³C(α) spin with ¹³C(α) steady state magnetization. The principle is demonstrated for the (HCA)CONH experiment, resulting in 1.6-fold sensitivity enhancement compared to the HN(CA)CO experiment. Furthermore, other versions of the (HCA)CONH experiment were evaluated, including a novel experiment with spin- locking of transverse ¹³C-¹H two-spin coherence, and a cross-correlation compensated (CA)CONH experiment which starts from ¹³C rather than ¹H magnetization.

Original language	English
Pages (from-to)	229-233
Number of pages	5
Journal	Journal of Biomolecular NMR
Volume	16
Issue number	3
DOIs	https://doi.org/10.1023/A:1008308106108
Publication status	Published - Mar 2000
Externally published	Yes

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title = "Sensitivity enhancement in (HCA)CONH experiments",

abstract = "A novel sensitivity-enhancement technique is proposed for experiments which correlate protein backbone resonances and start with magnetization from 13C(α)-1H(α) groups. The technique is based on replenishing magnetization lost by dipole-CSA cross-correlated relaxation of the 13C(α) spin with 13C(α) steady state magnetization. The principle is demonstrated for the (HCA)CONH experiment, resulting in 1.6-fold sensitivity enhancement compared to the HN(CA)CO experiment. Furthermore, other versions of the (HCA)CONH experiment were evaluated, including a novel experiment with spin- locking of transverse 13C-1H two-spin coherence, and a cross-correlation compensated (CA)CONH experiment which starts from 13C rather than 1H magnetization.",

keywords = "(CA)CONH, (HCA)CONH, Cross-correlation compensation, FMN-binding protein, HN(CA)CO, Ubiquitin",

author = "Rutger Folmer and Gottfried Otting",

year = "2000",

month = mar,

doi = "10.1023/A:1008308106108",

language = "English",

volume = "16",

pages = "229--233",

journal = "Journal of Biomolecular NMR",

issn = "0925-2738",

publisher = "Springer Science+Business Media B.V.",

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T1 - Sensitivity enhancement in (HCA)CONH experiments

AU - Folmer, Rutger

AU - Otting, Gottfried

PY - 2000/3

Y1 - 2000/3

N2 - A novel sensitivity-enhancement technique is proposed for experiments which correlate protein backbone resonances and start with magnetization from 13C(α)-1H(α) groups. The technique is based on replenishing magnetization lost by dipole-CSA cross-correlated relaxation of the 13C(α) spin with 13C(α) steady state magnetization. The principle is demonstrated for the (HCA)CONH experiment, resulting in 1.6-fold sensitivity enhancement compared to the HN(CA)CO experiment. Furthermore, other versions of the (HCA)CONH experiment were evaluated, including a novel experiment with spin- locking of transverse 13C-1H two-spin coherence, and a cross-correlation compensated (CA)CONH experiment which starts from 13C rather than 1H magnetization.

AB - A novel sensitivity-enhancement technique is proposed for experiments which correlate protein backbone resonances and start with magnetization from 13C(α)-1H(α) groups. The technique is based on replenishing magnetization lost by dipole-CSA cross-correlated relaxation of the 13C(α) spin with 13C(α) steady state magnetization. The principle is demonstrated for the (HCA)CONH experiment, resulting in 1.6-fold sensitivity enhancement compared to the HN(CA)CO experiment. Furthermore, other versions of the (HCA)CONH experiment were evaluated, including a novel experiment with spin- locking of transverse 13C-1H two-spin coherence, and a cross-correlation compensated (CA)CONH experiment which starts from 13C rather than 1H magnetization.

KW - (CA)CONH

KW - (HCA)CONH

KW - Cross-correlation compensation

KW - FMN-binding protein

KW - HN(CA)CO

KW - Ubiquitin

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DO - 10.1023/A:1008308106108

M3 - Article

SN - 0925-2738

VL - 16

SP - 229

EP - 233

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

IS - 3

ER -

Sensitivity enhancement in (HCA)CONH experiments

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