@inbook{f9b85b68b5324216ac9eced12e80926d,
title = "Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes",
abstract = "Sequential ChIP (ChIP-reChIP) enables the characterization of the same nucleosome for two different types of modifications or histone subtypes. Here, we describe a ChIP-reChIP protocol to identify a heterotypic (asymmetric) H2A.Z–H2A-containing nucleosome. In this method, following MNase digestion of chromatin to mostly a mononucleosome fraction, H2A.Z-containing nucleosomes are first immunoprecipitated using affinity purified anti-H2A.Z antibodies. This H2A.Z-containing nucleosome fraction is then subsequently immunoprecipitated using anti-H2A affinity purified antibodies to yield an enriched population of heterotypic H2A.Z–H2A containing nucleosomes. This protocol can be adopted to investigate any pair-wise combination of any histone variant, histone posttranslational modification, or any other protein that binds to a modified nucleosome.",
keywords = "Affinity purified antibodies, Chromatin immunoprecipitation, H2A, H2A.Z, Heterotypic nucleosomes, Histone variants, Nucleosomes",
author = "Maxim Nekrasov and Tremethick, {David J.}",
note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1597-3_8",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "147--161",
booktitle = "Methods in Molecular Biology",
}