Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water-Ethanol Mixtures

Nikolai F. Bunkin; Nikolai F. Bunkin; Alexey V. Shkirin; Alexey V. Shkirin; Barry W. Ninham; Sergey N. Chirikov; Leonid L. Chaikov; Nikita V. Penkov; Valeriy A. Kozlov; Sergey V. Gudkov

doi:10.1021/acsomega.0c01444

Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water-Ethanol Mixtures

Nikolai F. Bunkin^*, Nikolai F. Bunkin^*, Alexey V. Shkirin, Alexey V. Shkirin, Barry W. Ninham, Sergey N. Chirikov, Leonid L. Chaikov, Nikita V. Penkov, Valeriy A. Kozlov, Sergey V. Gudkov

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

Structural characterization by three complementary methods of laser diagnostics (dynamic light scattering, laser phase microscopy, and laser polarimetric scatterometry) has established that shaking of immunoglobulin G (IgG) dispersions in water and ethanol-water mixtures (36.7 vol %) results in two effects. First, it intensifies the aggregation of IgG macromolecules. Second, it generates bubbles with a size range that is different in each solvent. The aggregation is enhanced in ethanol-water mixtures because of IgG denaturation. IgG aggregates have a size of ∼300 nm in water and ∼900 nm in ethanol-water mixtures. The flotation of IgG is much more efficient in water. This can be explained by a better adsorption of IgG particles (molecules and aggregates) on bubbles in water as compared to ethanol-water mixtures. Bulk nanobubbles and their association with IgG aggregates were visualized by laser phase microscopy in water but were not detected in ethanol-water mixtures. Therefore, the nanobubble flotation mechanism for IgG aggregates acting in water is not feasible for ethanol-water mixtures.

Original language	English
Pages (from-to)	14689-14701
Number of pages	13
Journal	ACS Omega
Volume	5
Issue number	24
DOIs	https://doi.org/10.1021/acsomega.0c01444
Publication status	Published - 23 Jun 2020

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@article{6ffa67d5ffca4e3e89ee4cfdca6900b1,

title = "Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water-Ethanol Mixtures",

abstract = "Structural characterization by three complementary methods of laser diagnostics (dynamic light scattering, laser phase microscopy, and laser polarimetric scatterometry) has established that shaking of immunoglobulin G (IgG) dispersions in water and ethanol-water mixtures (36.7 vol %) results in two effects. First, it intensifies the aggregation of IgG macromolecules. Second, it generates bubbles with a size range that is different in each solvent. The aggregation is enhanced in ethanol-water mixtures because of IgG denaturation. IgG aggregates have a size of ∼300 nm in water and ∼900 nm in ethanol-water mixtures. The flotation of IgG is much more efficient in water. This can be explained by a better adsorption of IgG particles (molecules and aggregates) on bubbles in water as compared to ethanol-water mixtures. Bulk nanobubbles and their association with IgG aggregates were visualized by laser phase microscopy in water but were not detected in ethanol-water mixtures. Therefore, the nanobubble flotation mechanism for IgG aggregates acting in water is not feasible for ethanol-water mixtures.",

author = "Bunkin, {Nikolai F.} and Bunkin, {Nikolai F.} and Shkirin, {Alexey V.} and Shkirin, {Alexey V.} and Ninham, {Barry W.} and Chirikov, {Sergey N.} and Chaikov, {Leonid L.} and Penkov, {Nikita V.} and Kozlov, {Valeriy A.} and Gudkov, {Sergey V.}",

note = "Publisher Copyright: {\textcopyright} 2020 American Chemical Society.",

year = "2020",

month = jun,

day = "23",

doi = "10.1021/acsomega.0c01444",

language = "English",

volume = "5",

pages = "14689--14701",

journal = "ACS Omega",

issn = "2470-1343",

publisher = "American Chemical Society",

number = "24",

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TY - JOUR

T1 - Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions

T2 - Differences between Water and Water-Ethanol Mixtures

AU - Bunkin, Nikolai F.

AU - Shkirin, Alexey V.

AU - Ninham, Barry W.

AU - Chirikov, Sergey N.

AU - Chaikov, Leonid L.

AU - Penkov, Nikita V.

AU - Kozlov, Valeriy A.

AU - Gudkov, Sergey V.

PY - 2020/6/23

Y1 - 2020/6/23

N2 - Structural characterization by three complementary methods of laser diagnostics (dynamic light scattering, laser phase microscopy, and laser polarimetric scatterometry) has established that shaking of immunoglobulin G (IgG) dispersions in water and ethanol-water mixtures (36.7 vol %) results in two effects. First, it intensifies the aggregation of IgG macromolecules. Second, it generates bubbles with a size range that is different in each solvent. The aggregation is enhanced in ethanol-water mixtures because of IgG denaturation. IgG aggregates have a size of ∼300 nm in water and ∼900 nm in ethanol-water mixtures. The flotation of IgG is much more efficient in water. This can be explained by a better adsorption of IgG particles (molecules and aggregates) on bubbles in water as compared to ethanol-water mixtures. Bulk nanobubbles and their association with IgG aggregates were visualized by laser phase microscopy in water but were not detected in ethanol-water mixtures. Therefore, the nanobubble flotation mechanism for IgG aggregates acting in water is not feasible for ethanol-water mixtures.

AB - Structural characterization by three complementary methods of laser diagnostics (dynamic light scattering, laser phase microscopy, and laser polarimetric scatterometry) has established that shaking of immunoglobulin G (IgG) dispersions in water and ethanol-water mixtures (36.7 vol %) results in two effects. First, it intensifies the aggregation of IgG macromolecules. Second, it generates bubbles with a size range that is different in each solvent. The aggregation is enhanced in ethanol-water mixtures because of IgG denaturation. IgG aggregates have a size of ∼300 nm in water and ∼900 nm in ethanol-water mixtures. The flotation of IgG is much more efficient in water. This can be explained by a better adsorption of IgG particles (molecules and aggregates) on bubbles in water as compared to ethanol-water mixtures. Bulk nanobubbles and their association with IgG aggregates were visualized by laser phase microscopy in water but were not detected in ethanol-water mixtures. Therefore, the nanobubble flotation mechanism for IgG aggregates acting in water is not feasible for ethanol-water mixtures.

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U2 - 10.1021/acsomega.0c01444

DO - 10.1021/acsomega.0c01444

M3 - Article

SN - 2470-1343

VL - 5

SP - 14689

EP - 14701

JO - ACS Omega

JF - ACS Omega

IS - 24

ER -

Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water-Ethanol Mixtures

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