Significance of short chain fatty acid transport by members of the monocarboxylate transporter family (MCT)

Ivano Moschen, Angelika Bröer, Sandra Galić, Florian Lang, Stefan Bröer*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    59 Citations (Scopus)

    Abstract

    Metabolism of short-chain fatty acids (SCFA) in the brain, particularly that of acetate, appears to occur mainly in astrocytes. The differential use has been attributed to transport, but the extent to which transmembrane movement of SCFA is mediated by transporters has not been investigated systematically. Here we tested the possible contribution of monocarboxylate transporters to SCFA uptake by measuring fluxes with labelled compounds and by following changes of the intracellular pH in Xenopus laevis oocytes expressing the isoforms MCT1, MCT2 or MCT4. All isoforms mediated significant transport of acetate. Formate, however, was transported only by MCT1. The contribution of MCT1 to SCFA transport was determined by using phloretin as a high-affinity inhibitor, which allowed a paired comparison of oocytes with and without active MCT1.

    Original languageEnglish
    Pages (from-to)2562-2568
    Number of pages7
    JournalNeurochemical Research
    Volume37
    Issue number11
    DOIs
    Publication statusPublished - Nov 2012

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