Simultaneous multi-site two-photon photostimulation in three dimensions

Mary Ann Go; Christian Stricker; Stephen Redman; Hans A. Bachor; Vincent Ricardo Daria

doi:10.1002/jbio.201100101

Simultaneous multi-site two-photon photostimulation in three dimensions

Mary Ann Go, Christian Stricker, Stephen Redman, Hans A. Bachor, Vincent Ricardo Daria^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

We demonstrate simultaneous multi-site two-photon photolysis of caged neurotransmitters with close to diffraction-limited resolution in all three dimensions (3D). We use holographic projection of multiple focal spots, which allows full control over the 3D positions of uncaging sites with a high degree of localized excitation. Our system incorporates a two-photon imaging setup to visualize the 3D morphology of the neurons in order to accurately determine the photostimulation sites. We show its application to studies of synaptic integration by performing simultaneous and controlled glutamate delivery at multiple locations on dendritic trees.

Original language	English
Pages (from-to)	745-753
Number of pages	9
Journal	Journal of Biophotonics
Volume	5
Issue number	10
DOIs	https://doi.org/10.1002/jbio.201100101
Publication status	Published - Oct 2012

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10.1002/jbio.201100101

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title = "Simultaneous multi-site two-photon photostimulation in three dimensions",

abstract = "We demonstrate simultaneous multi-site two-photon photolysis of caged neurotransmitters with close to diffraction-limited resolution in all three dimensions (3D). We use holographic projection of multiple focal spots, which allows full control over the 3D positions of uncaging sites with a high degree of localized excitation. Our system incorporates a two-photon imaging setup to visualize the 3D morphology of the neurons in order to accurately determine the photostimulation sites. We show its application to studies of synaptic integration by performing simultaneous and controlled glutamate delivery at multiple locations on dendritic trees.",

keywords = "Holographic projection, Synaptic integration, Two-photon imaging, Two-photon photolysis",

author = "Go, {Mary Ann} and Christian Stricker and Stephen Redman and Bachor, {Hans A.} and Daria, {Vincent Ricardo}",

year = "2012",

month = oct,

doi = "10.1002/jbio.201100101",

language = "English",

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journal = "Journal of Biophotonics",

issn = "1864-063X",

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T1 - Simultaneous multi-site two-photon photostimulation in three dimensions

AU - Go, Mary Ann

AU - Stricker, Christian

AU - Redman, Stephen

AU - Bachor, Hans A.

AU - Daria, Vincent Ricardo

PY - 2012/10

Y1 - 2012/10

N2 - We demonstrate simultaneous multi-site two-photon photolysis of caged neurotransmitters with close to diffraction-limited resolution in all three dimensions (3D). We use holographic projection of multiple focal spots, which allows full control over the 3D positions of uncaging sites with a high degree of localized excitation. Our system incorporates a two-photon imaging setup to visualize the 3D morphology of the neurons in order to accurately determine the photostimulation sites. We show its application to studies of synaptic integration by performing simultaneous and controlled glutamate delivery at multiple locations on dendritic trees.

AB - We demonstrate simultaneous multi-site two-photon photolysis of caged neurotransmitters with close to diffraction-limited resolution in all three dimensions (3D). We use holographic projection of multiple focal spots, which allows full control over the 3D positions of uncaging sites with a high degree of localized excitation. Our system incorporates a two-photon imaging setup to visualize the 3D morphology of the neurons in order to accurately determine the photostimulation sites. We show its application to studies of synaptic integration by performing simultaneous and controlled glutamate delivery at multiple locations on dendritic trees.

KW - Holographic projection

KW - Synaptic integration

KW - Two-photon imaging

KW - Two-photon photolysis

UR - http://www.scopus.com/inward/record.url?scp=84861953358&partnerID=8YFLogxK

U2 - 10.1002/jbio.201100101

DO - 10.1002/jbio.201100101

M3 - Article

SN - 1864-063X

VL - 5

SP - 745

EP - 753

JO - Journal of Biophotonics

JF - Journal of Biophotonics

IS - 10

ER -

Simultaneous multi-site two-photon photostimulation in three dimensions

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