Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice

My Vo Thi Tra; Xiaojia Yin; Ishita Bajal; Christian Paolo Balahadia; William Paul Quick; Anindya Bandyopadhyay

doi:10.1007/978-1-0716-1068-8_9

Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice

My Vo Thi Tra, Xiaojia Yin, Ishita Bajal, Christian Paolo Balahadia, William Paul Quick, Anindya Bandyopadhyay^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Citations (Scopus)

Abstract

The fast-moving CRISPR technology has allowed plant scientists to manipulate plant genomes in a targeted manner. So far, most of the applications were focused on gene knocking out by creating indels. However, more precise genome editing tools are demanded to assist the introduction of functional single nucleotide polymorphisms (SNPs) in breeding programs. The CRISPR base editing tools were developed to meet this need. In this chapter, we present a cytidine deaminase base editing method for editing the point mutations that control the grain size and seed coat color in rice.

Original language	English
Title of host publication	Rice Genome Engineering and Gene Editing
Subtitle of host publication	Methods and Protocols
Editors	Anindya Bandyopadhyay, Roger Thilmony
Place of Publication	New York
Publisher	Humana Press Inc.
Chapter	9
Pages	135-143
ISBN (Electronic)	978-1-0716-1068-8
ISBN (Print)	978-1-0716-1067-1
DOIs	https://doi.org/10.1007/978-1-0716-1068-8_9
Publication status	Published - 2021
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2238
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Access to Document

10.1007/978-1-0716-1068-8_9

Cite this

Tra, M. V. T., Yin, X., Bajal, I., Balahadia, C. P., Quick, W. P., & Bandyopadhyay, A. (2021). Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice. In A. Bandyopadhyay, & R. Thilmony (Eds.), Rice Genome Engineering and Gene Editing: Methods and Protocols (pp. 135-143). (Methods in Molecular Biology; Vol. 2238). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-1068-8_9

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abstract = "The fast-moving CRISPR technology has allowed plant scientists to manipulate plant genomes in a targeted manner. So far, most of the applications were focused on gene knocking out by creating indels. However, more precise genome editing tools are demanded to assist the introduction of functional single nucleotide polymorphisms (SNPs) in breeding programs. The CRISPR base editing tools were developed to meet this need. In this chapter, we present a cytidine deaminase base editing method for editing the point mutations that control the grain size and seed coat color in rice.",

keywords = "Base editing, CRISPR, Cytidine deaminase, Rice",

author = "Tra, \{My Vo Thi\} and Xiaojia Yin and Ishita Bajal and Balahadia, \{Christian Paolo\} and Quick, \{William Paul\} and Anindya Bandyopadhyay",

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doi = "10.1007/978-1-0716-1068-8\_9",

language = "English",

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series = "Methods in Molecular Biology",

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Tra, MVT, Yin, X, Bajal, I, Balahadia, CP, Quick, WP & Bandyopadhyay, A 2021, Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice. in A Bandyopadhyay & R Thilmony (eds), Rice Genome Engineering and Gene Editing: Methods and Protocols. Methods in Molecular Biology, vol. 2238, Humana Press Inc., New York, pp. 135-143. https://doi.org/10.1007/978-1-0716-1068-8_9

Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice. / Tra, My Vo Thi; Yin, Xiaojia; Bajal, Ishita et al.
Rice Genome Engineering and Gene Editing: Methods and Protocols. ed. / Anindya Bandyopadhyay; Roger Thilmony. New York: Humana Press Inc., 2021. p. 135-143 (Methods in Molecular Biology; Vol. 2238).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice

AU - Tra, My Vo Thi

AU - Yin, Xiaojia

AU - Bajal, Ishita

AU - Balahadia, Christian Paolo

AU - Quick, William Paul

AU - Bandyopadhyay, Anindya

PY - 2021

Y1 - 2021

N2 - The fast-moving CRISPR technology has allowed plant scientists to manipulate plant genomes in a targeted manner. So far, most of the applications were focused on gene knocking out by creating indels. However, more precise genome editing tools are demanded to assist the introduction of functional single nucleotide polymorphisms (SNPs) in breeding programs. The CRISPR base editing tools were developed to meet this need. In this chapter, we present a cytidine deaminase base editing method for editing the point mutations that control the grain size and seed coat color in rice.

AB - The fast-moving CRISPR technology has allowed plant scientists to manipulate plant genomes in a targeted manner. So far, most of the applications were focused on gene knocking out by creating indels. However, more precise genome editing tools are demanded to assist the introduction of functional single nucleotide polymorphisms (SNPs) in breeding programs. The CRISPR base editing tools were developed to meet this need. In this chapter, we present a cytidine deaminase base editing method for editing the point mutations that control the grain size and seed coat color in rice.

KW - Base editing

KW - CRISPR

KW - Cytidine deaminase

KW - Rice

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SN - 978-1-0716-1067-1

T3 - Methods in Molecular Biology

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Tra MVT, Yin X, Bajal I, Balahadia CP, Quick WP, Bandyopadhyay A. Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice. In Bandyopadhyay A, Thilmony R, editors, Rice Genome Engineering and Gene Editing: Methods and Protocols. New York: Humana Press Inc. 2021. p. 135-143. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-1068-8_9

Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice

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