Site-directed mutagenesis of dienelactone hydrolase produces dienelactone isomerase

Ian Walker; Christopher J. Easton; David L. Ollis

doi:10.1039/b000365o

Site-directed mutagenesis of dienelactone hydrolase produces dienelactone isomerase

Ian Walker, Christopher J. Easton^*, David L. Ollis

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Replacing the active site Cys-123 of dienelactone hydrolase with Ser completely changes the catalysis displayed by the protein, from hydrolysis of the substrate E- and Z-dienelactones to maleylacetate by the native enzyme, to interconversion of the substrates by the mutant, dienelactone isomerase.

Original language	English
Pages (from-to)	671-672
Number of pages	2
Journal	Chemical Communications
Issue number	8
DOIs	https://doi.org/10.1039/b000365o
Publication status	Published - 21 Apr 2000

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title = "Site-directed mutagenesis of dienelactone hydrolase produces dienelactone isomerase",

abstract = "Replacing the active site Cys-123 of dienelactone hydrolase with Ser completely changes the catalysis displayed by the protein, from hydrolysis of the substrate E- and Z-dienelactones to maleylacetate by the native enzyme, to interconversion of the substrates by the mutant, dienelactone isomerase.",

author = "Ian Walker and Easton, {Christopher J.} and Ollis, {David L.}",

year = "2000",

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AU - Walker, Ian

AU - Easton, Christopher J.

AU - Ollis, David L.

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Y1 - 2000/4/21

N2 - Replacing the active site Cys-123 of dienelactone hydrolase with Ser completely changes the catalysis displayed by the protein, from hydrolysis of the substrate E- and Z-dienelactones to maleylacetate by the native enzyme, to interconversion of the substrates by the mutant, dienelactone isomerase.

AB - Replacing the active site Cys-123 of dienelactone hydrolase with Ser completely changes the catalysis displayed by the protein, from hydrolysis of the substrate E- and Z-dienelactones to maleylacetate by the native enzyme, to interconversion of the substrates by the mutant, dienelactone isomerase.

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U2 - 10.1039/b000365o

DO - 10.1039/b000365o

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JO - Chemical Communications

JF - Chemical Communications

IS - 8

ER -

Site-directed mutagenesis of dienelactone hydrolase produces dienelactone isomerase

Abstract

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