Software package for off-Axis digital holographic microscopy imaging processing

Tienan Xu*, Xuefei He, Zhiduo Zhang, Samantha Montague, Elizabeth Gardiner, Woie Ming Lee

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    Digital Holographic Microscopy (DHM) has emerged as a powerful imaging tool due to its ability to provide quantitative sample morphological data in a label-free manner. Off-Axis holography further opens possibilities of numerical approaches to image analysis and data processing. However, this involves computationally heavy workflows that limit the tool's usability in biomedical research. In this paper, a software package is developed for off-Axis hologram reconstruction and processing. The software can perform a variety of bioimaging operations including imaging, region of interest selection, automated reconstruction, and data extraction, with a user-friendly graphical user interface. Originally programmed in MATLAB, the software can be installed to use in common lab-based Microsoft Windows computers. The software can be easily adopted to work with customized off-Axis DHM setups, aiming to increase the efficiency of DHM's bioimaging workflow in the community. To demonstrate this software, blood platelet experiments were conducted to show quantitative volume change of thrombus formation.

    Original languageEnglish
    Title of host publicationBiophotonics Australasia 2019
    EditorsEwa M. Goldys, Brant C. Gibson
    PublisherSPIE
    ISBN (Electronic)9781510631441
    DOIs
    Publication statusPublished - 2019
    EventBiophotonics Australasia 2019 - Melbourne, Australia
    Duration: 9 Dec 201912 Dec 2019

    Publication series

    NameProceedings of SPIE - The International Society for Optical Engineering
    Volume11202
    ISSN (Print)0277-786X
    ISSN (Electronic)1996-756X

    Conference

    ConferenceBiophotonics Australasia 2019
    Country/TerritoryAustralia
    CityMelbourne
    Period9/12/1912/12/19

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