Spectroscopic selection of distance measurements in a protein dimer with mixed nitroxide and Gd 3+ spin labels

Ilia Kaminker, Hiromasa Yagi, Thomas Huber, Akiva Feintuch, Gottfried Otting*, Daniella Goldfarb

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    68 Citations (Scopus)

    Abstract

    The pulse DEER (Double Electron-Electron Resonance) technique is frequently applied for measuring nanometer distances between specific sites in biological macromolecules. In this work we extend the applicability of this method to high field distance measurements in a protein assembly with mixed spin labels, i.e. a nitroxide spin label and a Gd 3+ tag. We demonstrate the possibility of spectroscopic selection of distance distributions between two nitroxide spin labels, a nitroxide spin label and a Gd 3+ ion, and two Gd 3+ ions. Gd 3+-nitroxide DEER measurements possess high potential for W-band long range distance measurements (6 nm) by combining high sensitivity with ease of data analysis, subject to some instrumental improvements.

    Original languageEnglish
    Pages (from-to)4355-4358
    Number of pages4
    JournalPhysical Chemistry Chemical Physics
    Volume14
    Issue number13
    DOIs
    Publication statusPublished - 7 Apr 2012

    Fingerprint

    Dive into the research topics of 'Spectroscopic selection of distance measurements in a protein dimer with mixed nitroxide and Gd 3+ spin labels'. Together they form a unique fingerprint.

    Cite this