Spontaneous mutation at position 114 in H-2K(d) affects cytotoxic T cell responses to influenza virus infection

Arno Müllbacher*, Mario Lobigs, Jonathan W. Yewdell, Jack R. Bennink, Ron Tha Hla, Robert V. Blanden

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Vaccinia virus (VV)-encoded MHC class I K(d) molecules which differ by a single amino acid change from glutamine (K(dw), wild type) to histidine (K(dm), mutant) at position 114 located in the floor of the peptide binding groove were compared in terms of peptide binding and cytotoxic T (Tc) cell recognition. Most anti-viral Tc cells were nor affected or only marginally affected. However, the K(dm) molecule did not detectably present the immunodominant peptide (NPP147-155) of influenza virus nucleoprotein (NP), encoded by the full-length NP gene either in influenza A virus or recombinant W. This defect could be overcome by using exogenous synthetic NPP147-155 or translation from a minigene encoding NPP147-155 in W. K(dw) presented NPP147-155 encoded by the full-length NP) gene, but K(dw)-NPP147-155 complexes were at least 100-fold less abundant than after translation from a minigene.

    Original languageEnglish
    Pages (from-to)1228-1234
    Number of pages7
    JournalEuropean Journal of Immunology
    Volume29
    Issue number4
    DOIs
    Publication statusPublished - 1999

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