Steady-state chlorophyll fluorescence (Fs) measurements as a tool to follow variations of net CO2 assimilation and stomatal conductance during water-stress in C3 plants

Jaume Flexas*, José Mariano Escalona, Sebastian Evain, Javier Gulías, Ismaël Moya, Charles Barry Osmond, Hipólito Medrano

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    279 Citations (Scopus)

    Abstract

    Water stress experiments were performed with grapevines (Vitis vinifera L.) and other C3 plants in the field, in potted plants in the laboratory, and with detached leaves. It was found that, in all cases, the ratio of steady state chlorophyll fluorescence (Fs) normalized to dark-adapted intrinsic fluorescence (Fo) inversely correlated with non-photochemical quenching (NPQ). Also, at high irradiance, the ratio Fs/Fo was positively correlated with CO2 assimilation in air, with electron transport rate calculated from fluorescence, and with stomatal conductance, but no clear correlation was observed with qP. The significance of these relationships is discussed. The ratio Fs/Fo, measured with a portable instrument (PAM-2000) or with a remote sensing FIPAM system, provides a good method for the early detection of water stress, and may become a useful guide to irrigation requirements.

    Original languageEnglish
    Pages (from-to)231-240
    Number of pages10
    JournalPhysiologia Plantarum
    Volume114
    Issue number2
    DOIs
    Publication statusPublished - 2002

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