TY - JOUR
T1 - Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
AU - Hiebert, Colin W.
AU - Moscou, Matthew J.
AU - Hewitt, Tim
AU - Steuernagel, Burkhard
AU - Hernández-Pinzón, Inma
AU - Green, Phon
AU - Pujol, Vincent
AU - Zhang, Peng
AU - Rouse, Matthew N.
AU - Jin, Yue
AU - McIntosh, Robert A.
AU - Upadhyaya, Narayana
AU - Zhang, Jianping
AU - Bhavani, Sridhar
AU - Vrána, Jan
AU - Karafiátová, Miroslava
AU - Huang, Li
AU - Fetch, Tom
AU - Doležel, Jaroslav
AU - Wulff, Brande B.H.
AU - Lagudah, Evans
AU - Spielmeyer, Wolfgang
N1 - Publisher Copyright:
© 2020, Crown.
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.
AB - Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.
UR - http://www.scopus.com/inward/record.url?scp=85080992910&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-14937-2
DO - 10.1038/s41467-020-14937-2
M3 - Article
SN - 2041-1723
VL - 11
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1123
ER -