Structural and evolutionary approaches to the design and optimization of fluorescence-based small molecule biosensors

Joe A. Kaczmarski; Joshua A. Mitchell; Matthew A. Spence; Vanessa Vongsouthi; Colin J. Jackson

doi:10.1016/j.sbi.2019.01.013

Structural and evolutionary approaches to the design and optimization of fluorescence-based small molecule biosensors

Joe A. Kaczmarski, Joshua A. Mitchell, Matthew A. Spence, Vanessa Vongsouthi, Colin J. Jackson

Research output: Contribution to journal › Review article › peer-review

24 Citations (Scopus)

Abstract

Biosensors that selectively report on the presence of specific small molecule analytes have applications in many fields of research, medicine and biotechnology. Here, we review recent advances and emerging approaches in the design and optimisation of genetically encoded fluorescence-based small molecule biosensors. We discuss how natural sensory proteins can be exploited to produce novel biosensors and the strategies for optimizing ligand specificity and fluorescence readout. Finally, we provide insight into high-throughput sensor optimisation and discuss the challenges that are faced when designing novel biosensors.

Original language	English
Pages (from-to)	31-38
Number of pages	8
Journal	Current Opinion in Structural Biology
Volume	57
DOIs	https://doi.org/10.1016/j.sbi.2019.01.013
Publication status	Published - Aug 2019

Access to Document

10.1016/j.sbi.2019.01.013

Cite this

@article{5b7dfaace17645a49c84c0ef28cac8ea,

title = "Structural and evolutionary approaches to the design and optimization of fluorescence-based small molecule biosensors",

abstract = "Biosensors that selectively report on the presence of specific small molecule analytes have applications in many fields of research, medicine and biotechnology. Here, we review recent advances and emerging approaches in the design and optimisation of genetically encoded fluorescence-based small molecule biosensors. We discuss how natural sensory proteins can be exploited to produce novel biosensors and the strategies for optimizing ligand specificity and fluorescence readout. Finally, we provide insight into high-throughput sensor optimisation and discuss the challenges that are faced when designing novel biosensors.",

author = "Kaczmarski, {Joe A.} and Mitchell, {Joshua A.} and Spence, {Matthew A.} and Vanessa Vongsouthi and Jackson, {Colin J.}",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = aug,

doi = "10.1016/j.sbi.2019.01.013",

language = "English",

volume = "57",

pages = "31--38",

journal = "Current Opinion in Structural Biology",

issn = "0959-440X",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Structural and evolutionary approaches to the design and optimization of fluorescence-based small molecule biosensors

AU - Kaczmarski, Joe A.

AU - Mitchell, Joshua A.

AU - Spence, Matthew A.

AU - Vongsouthi, Vanessa

AU - Jackson, Colin J.

PY - 2019/8

Y1 - 2019/8

N2 - Biosensors that selectively report on the presence of specific small molecule analytes have applications in many fields of research, medicine and biotechnology. Here, we review recent advances and emerging approaches in the design and optimisation of genetically encoded fluorescence-based small molecule biosensors. We discuss how natural sensory proteins can be exploited to produce novel biosensors and the strategies for optimizing ligand specificity and fluorescence readout. Finally, we provide insight into high-throughput sensor optimisation and discuss the challenges that are faced when designing novel biosensors.

AB - Biosensors that selectively report on the presence of specific small molecule analytes have applications in many fields of research, medicine and biotechnology. Here, we review recent advances and emerging approaches in the design and optimisation of genetically encoded fluorescence-based small molecule biosensors. We discuss how natural sensory proteins can be exploited to produce novel biosensors and the strategies for optimizing ligand specificity and fluorescence readout. Finally, we provide insight into high-throughput sensor optimisation and discuss the challenges that are faced when designing novel biosensors.

UR - http://www.scopus.com/inward/record.url?scp=85062147152&partnerID=8YFLogxK

U2 - 10.1016/j.sbi.2019.01.013

DO - 10.1016/j.sbi.2019.01.013

M3 - Review article

SN - 0959-440X

VL - 57

SP - 31

EP - 38

JO - Current Opinion in Structural Biology

JF - Current Opinion in Structural Biology

ER -

Structural and evolutionary approaches to the design and optimization of fluorescence-based small molecule biosensors

Abstract

Access to Document

Other files and links

Fingerprint

Cite this