Structural changes in the promoter region mediate transvection at the sn-glycerol-3-phosphate dehydrogenase gene of Drosophila melanogaster

J. B. Gibson; D. S. Reed; S. Bartoszewski; A. V. Wilks

doi:10.1023/a:1018707226595

Structural changes in the promoter region mediate transvection at the sn-glycerol-3-phosphate dehydrogenase gene of Drosophila melanogaster

J. B. Gibson^*, D. S. Reed, S. Bartoszewski, A. V. Wilks

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Trans effects at the sn-glycerol-3-phosphate dehydrogenase locus (Gpdh) of Drosophila melanogaster give rise to an increase in GPDH activity and mRNA from the wild-type allele in heterozygotes with some low-activity alleles. Either the low-activity alleles that induce the effect have a defective P- element inserted between the promoter and a downstream intronic enhancer element or the promoter region is deleted. The trans effect is pairing dependent, characteristic of transvection at some other loci. The defective P-elements that mediate transvection are located between the promoter and at least up to 6 bp downstream of the transcription start site. Transvection at Gpdh appears similar to the 'enhancer action in trans' mode at the yellow locus.

Original language	English
Pages (from-to)	301-315
Number of pages	15
Journal	Biochemical Genetics
Volume	37
Issue number	9-10
DOIs	https://doi.org/10.1023/a:1018707226595
Publication status	Published - 1999

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title = "Structural changes in the promoter region mediate transvection at the sn-glycerol-3-phosphate dehydrogenase gene of Drosophila melanogaster",

abstract = "Trans effects at the sn-glycerol-3-phosphate dehydrogenase locus (Gpdh) of Drosophila melanogaster give rise to an increase in GPDH activity and mRNA from the wild-type allele in heterozygotes with some low-activity alleles. Either the low-activity alleles that induce the effect have a defective P- element inserted between the promoter and a downstream intronic enhancer element or the promoter region is deleted. The trans effect is pairing dependent, characteristic of transvection at some other loci. The defective P-elements that mediate transvection are located between the promoter and at least up to 6 bp downstream of the transcription start site. Transvection at Gpdh appears similar to the 'enhancer action in trans' mode at the yellow locus.",

keywords = "Drosophila melanogaster, Enhancers, Gpdh, KP-element, Transvection",

author = "Gibson, \{J. B.\} and Reed, \{D. S.\} and S. Bartoszewski and Wilks, \{A. V.\}",

year = "1999",

doi = "10.1023/a:1018707226595",

language = "English",

volume = "37",

pages = "301--315",

journal = "Biochemical Genetics",

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T1 - Structural changes in the promoter region mediate transvection at the sn-glycerol-3-phosphate dehydrogenase gene of Drosophila melanogaster

AU - Gibson, J. B.

AU - Reed, D. S.

AU - Bartoszewski, S.

AU - Wilks, A. V.

PY - 1999

Y1 - 1999

N2 - Trans effects at the sn-glycerol-3-phosphate dehydrogenase locus (Gpdh) of Drosophila melanogaster give rise to an increase in GPDH activity and mRNA from the wild-type allele in heterozygotes with some low-activity alleles. Either the low-activity alleles that induce the effect have a defective P- element inserted between the promoter and a downstream intronic enhancer element or the promoter region is deleted. The trans effect is pairing dependent, characteristic of transvection at some other loci. The defective P-elements that mediate transvection are located between the promoter and at least up to 6 bp downstream of the transcription start site. Transvection at Gpdh appears similar to the 'enhancer action in trans' mode at the yellow locus.

AB - Trans effects at the sn-glycerol-3-phosphate dehydrogenase locus (Gpdh) of Drosophila melanogaster give rise to an increase in GPDH activity and mRNA from the wild-type allele in heterozygotes with some low-activity alleles. Either the low-activity alleles that induce the effect have a defective P- element inserted between the promoter and a downstream intronic enhancer element or the promoter region is deleted. The trans effect is pairing dependent, characteristic of transvection at some other loci. The defective P-elements that mediate transvection are located between the promoter and at least up to 6 bp downstream of the transcription start site. Transvection at Gpdh appears similar to the 'enhancer action in trans' mode at the yellow locus.

KW - Drosophila melanogaster

KW - Enhancers

KW - Gpdh

KW - KP-element

KW - Transvection

UR - https://www.scopus.com/pages/publications/0033451934

U2 - 10.1023/a:1018707226595

DO - 10.1023/a:1018707226595

M3 - Article

SN - 0006-2928

VL - 37

SP - 301

EP - 315

JO - Biochemical Genetics

JF - Biochemical Genetics

IS - 9-10

ER -

Structural changes in the promoter region mediate transvection at the sn-glycerol-3-phosphate dehydrogenase gene of Drosophila melanogaster

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