Structural insights into P-glycoprotein (ABCB1) by small angle X-ray scattering and electron crystallography

Christopher A. McDevitt; Chitra A. Shintre; J. Günter Grossmann; Naomi L. Pollock; Stephen M. Prince; Richard Callaghan; Robert C. Ford

doi:10.1016/j.febslet.2008.07.022

Structural insights into P-glycoprotein (ABCB1) by small angle X-ray scattering and electron crystallography

Christopher A. McDevitt, Chitra A. Shintre, J. Günter Grossmann, Naomi L. Pollock, Stephen M. Prince, Richard Callaghan, Robert C. Ford^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

P-glycoprotein (ABCB1) is an ATP-binding cassette protein that is associated with the acquisition of multi-drug resistance in cancer and the failure of chemotherapy in humans. Structural insights into this protein are described using a combination of small angle X-ray scattering data and cryo-electron crystallography data. We have compared the structures with bacterial homologues, and discuss the development of homology models for P-glycoprotein based on the bacterial Sav1866 structure.

Original language	English
Pages (from-to)	2950-2956
Number of pages	7
Journal	FEBS Letters
Volume	582
Issue number	19
DOIs	https://doi.org/10.1016/j.febslet.2008.07.022
Publication status	Published - 20 Aug 2008
Externally published	Yes

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10.1016/j.febslet.2008.07.022

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title = "Structural insights into P-glycoprotein (ABCB1) by small angle X-ray scattering and electron crystallography",

abstract = "P-glycoprotein (ABCB1) is an ATP-binding cassette protein that is associated with the acquisition of multi-drug resistance in cancer and the failure of chemotherapy in humans. Structural insights into this protein are described using a combination of small angle X-ray scattering data and cryo-electron crystallography data. We have compared the structures with bacterial homologues, and discuss the development of homology models for P-glycoprotein based on the bacterial Sav1866 structure.",

keywords = "ABCB1, Electron crystallography, Membrane protein, Multidrug resistance, Protein structure, SAXS",

author = "McDevitt, {Christopher A.} and Shintre, {Chitra A.} and {G{\"u}nter Grossmann}, J. and Pollock, {Naomi L.} and Prince, {Stephen M.} and Richard Callaghan and Ford, {Robert C.}",

year = "2008",

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doi = "10.1016/j.febslet.2008.07.022",

language = "English",

volume = "582",

pages = "2950--2956",

journal = "FEBS Letters",

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T1 - Structural insights into P-glycoprotein (ABCB1) by small angle X-ray scattering and electron crystallography

AU - McDevitt, Christopher A.

AU - Shintre, Chitra A.

AU - Günter Grossmann, J.

AU - Pollock, Naomi L.

AU - Prince, Stephen M.

AU - Callaghan, Richard

AU - Ford, Robert C.

PY - 2008/8/20

Y1 - 2008/8/20

N2 - P-glycoprotein (ABCB1) is an ATP-binding cassette protein that is associated with the acquisition of multi-drug resistance in cancer and the failure of chemotherapy in humans. Structural insights into this protein are described using a combination of small angle X-ray scattering data and cryo-electron crystallography data. We have compared the structures with bacterial homologues, and discuss the development of homology models for P-glycoprotein based on the bacterial Sav1866 structure.

AB - P-glycoprotein (ABCB1) is an ATP-binding cassette protein that is associated with the acquisition of multi-drug resistance in cancer and the failure of chemotherapy in humans. Structural insights into this protein are described using a combination of small angle X-ray scattering data and cryo-electron crystallography data. We have compared the structures with bacterial homologues, and discuss the development of homology models for P-glycoprotein based on the bacterial Sav1866 structure.

KW - ABCB1

KW - Electron crystallography

KW - Membrane protein

KW - Multidrug resistance

KW - Protein structure

KW - SAXS

UR - http://www.scopus.com/inward/record.url?scp=48749107151&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2008.07.022

DO - 10.1016/j.febslet.2008.07.022

M3 - Article

SN - 0014-5793

VL - 582

SP - 2950

EP - 2956

JO - FEBS Letters

JF - FEBS Letters

IS - 19

ER -

Structural insights into P-glycoprotein (ABCB1) by small angle X-ray scattering and electron crystallography

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