Structure-based rational design of a phosphotriesterase

Colin J. Jackson, Kahli Weir, Anthony Herlt, Jeevan Khurana, Tara D. Sutherland, Irene Horne, Christopher Easton, Robyn J. Russell, Colin Scott*, John G. Oakeshott

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    35 Citations (Scopus)

    Abstract

    In silico substrate docking of both stereoisomers of the pesticide chlorfenvinphos (CVP) in the phosphotriesterase from Agrobacterium radiobacter identified two residues (F131 and W132) that prevent productive substrate binding and cause stereospecificity. A variant (W131H/F132A) was designed that exhibited ca. 480-fold and 8-fold increases in the rate of Z-CVP and E-CVP hydrolysis, respectively, eliminating stereospecificity.

    Original languageEnglish
    Pages (from-to)5153-5156
    Number of pages4
    JournalApplied and Environmental Microbiology
    Volume75
    Issue number15
    DOIs
    Publication statusPublished - Aug 2009

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