TY - JOUR
T1 - Studies on the mechanism of the reaction between 5′-deoxyadenosylcobinamide and cyanide
AU - Brasch, Nicola E.
AU - Cregan, Andrew G.
AU - Vanselow, Mary E.
PY - 2002
Y1 - 2002
N2 - The reaction between 5′-deoxyadenosylcobinamide (AdoCbi+OH-) and cyanide (NaCN or tetrabutylammonium cyanide) to form dicyanocobinamide, adenine and 1-cyano-D-erythro-2,3-dihydroxy-4-pentenol has been studied by 1H NMR and UV-visible spectroscopies in aqueous solution at pH (pD) 11.0 and in 92% N,N-dimethylformamide (DMF)-8% D2O. Saturation kinetics are observed by UV-visible spectroscopy at high cyanide concentrations and data were fitted to a mechanism involving rapid formation of an (β-adenosyl)(α-cyano)cobinamide intermediate, (β-Ado)(α-CN)Cbi (KCN = 5.6 (H2O) and 6.9 M-1 (D2O), 25.0°C, I = 1.0 M (NaClO4); KCN = 4.6 (H2O) and 3.8 M-1 (D2O), 25.0°C, I = 2.0 M (NaClO4), KCN = 2.8 × 103 M-1, 25.0°C, I = 0.50 M ((TBA)ClO4)), followed by solvent-assisted, rate-determining cleavage of the Co-Ado bond of (β-Ado)(α-CN)Cbi (102k2 = 2.91 (H2O) and 2.17 s-1 (D2O), 25.0°C, I. = 1.0 M (NaClO4); 102k2 = 2.55 (H2O) and 2.45 s-1 (D2O), 25.0°C, I = 2.0 M (NaClO4), 105k2 = 6.7 s-1, 92% DMF-8% D2O, 25.0°C, I = 0.50 M ((TBA)ClO4)). Deviations in the plots of the observed rate constant versus cyanide concentration in aqueous solution at high cyanide concentrations is attributed to medium effects, and only CN-, not HCN, reacts with AdoCbiOH. Evidence for rapid formation of (β-adenosyl)(α-cyano)cobinamide from AdoCbiOH plus cyanide is also obtained by investigating the 1H NMR chemical shifts of the signals in the aromatic region immediately after mixing AdoCbiOH and cyanide as a function of cyanide concentration (D2O, 25.0°C; KCN′ = 5.7 and 3.3 M-1 at I = 1.0 (NaClO4) and 2.0 M (NaClO4), respectively). A comparison of rate and equilibria constants for the reaction between AdoCbiOH and 5′-deoxyadenosylcobalamin is made.
AB - The reaction between 5′-deoxyadenosylcobinamide (AdoCbi+OH-) and cyanide (NaCN or tetrabutylammonium cyanide) to form dicyanocobinamide, adenine and 1-cyano-D-erythro-2,3-dihydroxy-4-pentenol has been studied by 1H NMR and UV-visible spectroscopies in aqueous solution at pH (pD) 11.0 and in 92% N,N-dimethylformamide (DMF)-8% D2O. Saturation kinetics are observed by UV-visible spectroscopy at high cyanide concentrations and data were fitted to a mechanism involving rapid formation of an (β-adenosyl)(α-cyano)cobinamide intermediate, (β-Ado)(α-CN)Cbi (KCN = 5.6 (H2O) and 6.9 M-1 (D2O), 25.0°C, I = 1.0 M (NaClO4); KCN = 4.6 (H2O) and 3.8 M-1 (D2O), 25.0°C, I = 2.0 M (NaClO4), KCN = 2.8 × 103 M-1, 25.0°C, I = 0.50 M ((TBA)ClO4)), followed by solvent-assisted, rate-determining cleavage of the Co-Ado bond of (β-Ado)(α-CN)Cbi (102k2 = 2.91 (H2O) and 2.17 s-1 (D2O), 25.0°C, I. = 1.0 M (NaClO4); 102k2 = 2.55 (H2O) and 2.45 s-1 (D2O), 25.0°C, I = 2.0 M (NaClO4), 105k2 = 6.7 s-1, 92% DMF-8% D2O, 25.0°C, I = 0.50 M ((TBA)ClO4)). Deviations in the plots of the observed rate constant versus cyanide concentration in aqueous solution at high cyanide concentrations is attributed to medium effects, and only CN-, not HCN, reacts with AdoCbiOH. Evidence for rapid formation of (β-adenosyl)(α-cyano)cobinamide from AdoCbiOH plus cyanide is also obtained by investigating the 1H NMR chemical shifts of the signals in the aromatic region immediately after mixing AdoCbiOH and cyanide as a function of cyanide concentration (D2O, 25.0°C; KCN′ = 5.7 and 3.3 M-1 at I = 1.0 (NaClO4) and 2.0 M (NaClO4), respectively). A comparison of rate and equilibria constants for the reaction between AdoCbiOH and 5′-deoxyadenosylcobalamin is made.
UR - http://www.scopus.com/inward/record.url?scp=0036010407&partnerID=8YFLogxK
U2 - 10.1039/b109292h
DO - 10.1039/b109292h
M3 - Article
SN - 1470-479X
SP - 1287
EP - 1294
JO - Journal of the Chemical Society. Dalton Transactions
JF - Journal of the Chemical Society. Dalton Transactions
IS - 7
ER -