Abstract
A ganglioside GM1 probe bearing a dark-red fluorescent dye at the sphingosine moiety of the molecule was prepared by a convenient one-pot synthesis. The labeled GM1 permitted the detection of the natural ganglioside GM1 ligand Escherichia coli heat-labile enterotoxin subunit B (EtxB) in picomole quantities on a solid support. When an epitope mapping of several ganglioside binding proteins and protein fragments was performed by screening a cellulose membrane-bound synthetic library of 64 16mer peptides with the new probe, several peptides displaying ganglioside G M1 affinity could be identified. We consider the labeled glycolipid described herein a versatile tool for manifold biochemical investigations.
Original language | English |
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Pages (from-to) | 573-578 |
Number of pages | 6 |
Journal | Bioconjugate Chemistry |
Volume | 18 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2007 |