Temperature modulation of fatty acid profiles for biofuel production in nitrogen deprived Chlamydomonas reinhardtii

Gabriel O. James; Charles H. Hocart; Warwick Hillier; G. Dean Price; Michael A. Djordjevic

doi:10.1016/j.biortech.2012.09.090

Temperature modulation of fatty acid profiles for biofuel production in nitrogen deprived Chlamydomonas reinhardtii

Gabriel O. James, Charles H. Hocart, Warwick Hillier, G. Dean Price, Michael A. Djordjevic^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

This study investigated the changes in the fatty acid content and composition in the nitrogen-starved. Chlamydomonas reinhardtii starchless mutant, BAF-J5, grown at different temperatures. The optimal temperature for vegetative growth under nitrogen sufficient conditions was found to be 32 °C. Shifting temperature from 25 to 32 °C, in conjunction with nitrogen starvation, resulted in BAF-J5 storing the maximum quantity of fatty acid (76% of dry cell weight). Shifting to temperatures lower than 25 °C, reduced the total amount of stored fatty acid content and increased the level of desaturation in the fatty acids. The optimal fatty acid composition for biodiesel was at 32 °C. This study demonstrates how a critical environmental factor, such as temperature, can modulate the amount and composition of fatty acids under nitrogen deprivation and reduce the requirement for costly refining of biofuels.

Original language	English
Pages (from-to)	441-447
Number of pages	7
Journal	Bioresource Technology
Volume	127
DOIs	https://doi.org/10.1016/j.biortech.2012.09.090
Publication status	Published - Jan 2013

Access to Document

10.1016/j.biortech.2012.09.090

Cite this

@article{cf07f25745b94250b4fcdbea23e11fc0,

title = "Temperature modulation of fatty acid profiles for biofuel production in nitrogen deprived Chlamydomonas reinhardtii",

abstract = "This study investigated the changes in the fatty acid content and composition in the nitrogen-starved. Chlamydomonas reinhardtii starchless mutant, BAF-J5, grown at different temperatures. The optimal temperature for vegetative growth under nitrogen sufficient conditions was found to be 32 °C. Shifting temperature from 25 to 32 °C, in conjunction with nitrogen starvation, resulted in BAF-J5 storing the maximum quantity of fatty acid (76% of dry cell weight). Shifting to temperatures lower than 25 °C, reduced the total amount of stored fatty acid content and increased the level of desaturation in the fatty acids. The optimal fatty acid composition for biodiesel was at 32 °C. This study demonstrates how a critical environmental factor, such as temperature, can modulate the amount and composition of fatty acids under nitrogen deprivation and reduce the requirement for costly refining of biofuels.",

keywords = "Algae, Biofuel, Chlamydomonas reinhardtii, Fatty acid, Temperature",

author = "James, {Gabriel O.} and Hocart, {Charles H.} and Warwick Hillier and Price, {G. Dean} and Djordjevic, {Michael A.}",

year = "2013",

month = jan,

doi = "10.1016/j.biortech.2012.09.090",

language = "English",

volume = "127",

pages = "441--447",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Temperature modulation of fatty acid profiles for biofuel production in nitrogen deprived Chlamydomonas reinhardtii

AU - James, Gabriel O.

AU - Hocart, Charles H.

AU - Hillier, Warwick

AU - Price, G. Dean

AU - Djordjevic, Michael A.

PY - 2013/1

Y1 - 2013/1

N2 - This study investigated the changes in the fatty acid content and composition in the nitrogen-starved. Chlamydomonas reinhardtii starchless mutant, BAF-J5, grown at different temperatures. The optimal temperature for vegetative growth under nitrogen sufficient conditions was found to be 32 °C. Shifting temperature from 25 to 32 °C, in conjunction with nitrogen starvation, resulted in BAF-J5 storing the maximum quantity of fatty acid (76% of dry cell weight). Shifting to temperatures lower than 25 °C, reduced the total amount of stored fatty acid content and increased the level of desaturation in the fatty acids. The optimal fatty acid composition for biodiesel was at 32 °C. This study demonstrates how a critical environmental factor, such as temperature, can modulate the amount and composition of fatty acids under nitrogen deprivation and reduce the requirement for costly refining of biofuels.

AB - This study investigated the changes in the fatty acid content and composition in the nitrogen-starved. Chlamydomonas reinhardtii starchless mutant, BAF-J5, grown at different temperatures. The optimal temperature for vegetative growth under nitrogen sufficient conditions was found to be 32 °C. Shifting temperature from 25 to 32 °C, in conjunction with nitrogen starvation, resulted in BAF-J5 storing the maximum quantity of fatty acid (76% of dry cell weight). Shifting to temperatures lower than 25 °C, reduced the total amount of stored fatty acid content and increased the level of desaturation in the fatty acids. The optimal fatty acid composition for biodiesel was at 32 °C. This study demonstrates how a critical environmental factor, such as temperature, can modulate the amount and composition of fatty acids under nitrogen deprivation and reduce the requirement for costly refining of biofuels.

KW - Algae

KW - Biofuel

KW - Chlamydomonas reinhardtii

KW - Fatty acid

KW - Temperature

UR - http://www.scopus.com/inward/record.url?scp=84868305840&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2012.09.090

DO - 10.1016/j.biortech.2012.09.090

M3 - Article

SN - 0960-8524

VL - 127

SP - 441

EP - 447

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Temperature modulation of fatty acid profiles for biofuel production in nitrogen deprived Chlamydomonas reinhardtii

Abstract

Access to Document

Other files and links

Fingerprint

Cite this