Terahertz and far infrared Spectroscopy of alanine-rich peptides having variable ellipticity

Tao Ding; Ruoyu Li; J. Axel Zeitler; Thomas L. Huber; Lynn F. Gladden; Anton P.J. Middelberg; Robert J. Falconer

doi:10.1364/OE.18.027431

Terahertz and far infrared Spectroscopy of alanine-rich peptides having variable ellipticity

Tao Ding^*, Ruoyu Li, J. Axel Zeitler, Thomas L. Huber, Lynn F. Gladden, Anton P.J. Middelberg, Robert J. Falconer

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Terahertz spectra of four alanine-rich peptides with known secondary structures were studied by terahertz time domain spectroscopy (THz-TDS) and by Fourier transform infrared spectroscopy (FTIR) using a synchrotron light source and a liquid-helium cooled bolometer. At ambient temperatures the usable bandwidth was restricted to 0.2-1.5 THz by the absorbance of water. The existence of a solvation shell around the peptide in solution was observed and its size estimated to be between 11 and 17 Å. By cooling the peptide solution to 80 K in order to reduce the water absorbance the bandwidth was increased to 0.1-3.0 THz for both THz-TDS and FTIR. Spectra were consistent with monotonic absorbance of the peptide and the existence of a solid amorphous low density solvation shell.

Original language	English
Pages (from-to)	27431-27444
Number of pages	14
Journal	Optics Express
Volume	18
Issue number	26
DOIs	https://doi.org/10.1364/OE.18.027431
Publication status	Published - 20 Dec 2010

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title = "Terahertz and far infrared Spectroscopy of alanine-rich peptides having variable ellipticity",

abstract = "Terahertz spectra of four alanine-rich peptides with known secondary structures were studied by terahertz time domain spectroscopy (THz-TDS) and by Fourier transform infrared spectroscopy (FTIR) using a synchrotron light source and a liquid-helium cooled bolometer. At ambient temperatures the usable bandwidth was restricted to 0.2-1.5 THz by the absorbance of water. The existence of a solvation shell around the peptide in solution was observed and its size estimated to be between 11 and 17 {\AA}. By cooling the peptide solution to 80 K in order to reduce the water absorbance the bandwidth was increased to 0.1-3.0 THz for both THz-TDS and FTIR. Spectra were consistent with monotonic absorbance of the peptide and the existence of a solid amorphous low density solvation shell.",

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T1 - Terahertz and far infrared Spectroscopy of alanine-rich peptides having variable ellipticity

AU - Ding, Tao

AU - Li, Ruoyu

AU - Zeitler, J. Axel

AU - Huber, Thomas L.

AU - Gladden, Lynn F.

AU - Middelberg, Anton P.J.

AU - Falconer, Robert J.

PY - 2010/12/20

Y1 - 2010/12/20

N2 - Terahertz spectra of four alanine-rich peptides with known secondary structures were studied by terahertz time domain spectroscopy (THz-TDS) and by Fourier transform infrared spectroscopy (FTIR) using a synchrotron light source and a liquid-helium cooled bolometer. At ambient temperatures the usable bandwidth was restricted to 0.2-1.5 THz by the absorbance of water. The existence of a solvation shell around the peptide in solution was observed and its size estimated to be between 11 and 17 Å. By cooling the peptide solution to 80 K in order to reduce the water absorbance the bandwidth was increased to 0.1-3.0 THz for both THz-TDS and FTIR. Spectra were consistent with monotonic absorbance of the peptide and the existence of a solid amorphous low density solvation shell.

AB - Terahertz spectra of four alanine-rich peptides with known secondary structures were studied by terahertz time domain spectroscopy (THz-TDS) and by Fourier transform infrared spectroscopy (FTIR) using a synchrotron light source and a liquid-helium cooled bolometer. At ambient temperatures the usable bandwidth was restricted to 0.2-1.5 THz by the absorbance of water. The existence of a solvation shell around the peptide in solution was observed and its size estimated to be between 11 and 17 Å. By cooling the peptide solution to 80 K in order to reduce the water absorbance the bandwidth was increased to 0.1-3.0 THz for both THz-TDS and FTIR. Spectra were consistent with monotonic absorbance of the peptide and the existence of a solid amorphous low density solvation shell.

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DO - 10.1364/OE.18.027431

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JO - Optics Express

JF - Optics Express

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ER -

Terahertz and far infrared Spectroscopy of alanine-rich peptides having variable ellipticity

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