The chemotactic receptor EBI2 regulates the homeostasis, localization and immunological function of splenic dendritic cells

Dominique Gatto; Katherine Wood; Irina Caminschi; Danielle Murphy-Durland; Peter Schofield; Daniel Christ; Gunasegaran Karupiah; Robert Brink

doi:10.1038/ni.2555

The chemotactic receptor EBI2 regulates the homeostasis, localization and immunological function of splenic dendritic cells

Dominique Gatto, Katherine Wood, Irina Caminschi, Danielle Murphy-Durland, Peter Schofield, Daniel Christ, Gunasegaran Karupiah, Robert Brink^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

152 Citations (Scopus)

Abstract

Spleen-resident dendritic cell (DC) populations occupy sentinel positions for the capture and presentation of blood-borne antigens. Here we found a difference in expression of the chemotactic receptor EBI2 (GPR183) on splenic DC subsets and that EBI2 regulated the positioning and homeostasis of DCs in the spleen. EBI2 and its main ligand, 7α,25-OHC, were required for the generation of the splenic CD4⁺DC subset and the localization of DCs in bridging channels. Absence of EBI2 from DCs resulted in defects in both the activation of CD4⁺T cells and the induction of antibody responses. Regulated expression of EBI2 on DC populations is therefore critical for the generation and correct positioning of splenic DCs and the initiation of immune responses.

Original language	English
Pages (from-to)	446-453
Number of pages	8
Journal	Nature Immunology
Volume	14
Issue number	5
DOIs	https://doi.org/10.1038/ni.2555
Publication status	Published - May 2013

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abstract = "Spleen-resident dendritic cell (DC) populations occupy sentinel positions for the capture and presentation of blood-borne antigens. Here we found a difference in expression of the chemotactic receptor EBI2 (GPR183) on splenic DC subsets and that EBI2 regulated the positioning and homeostasis of DCs in the spleen. EBI2 and its main ligand, 7α,25-OHC, were required for the generation of the splenic CD4+DC subset and the localization of DCs in bridging channels. Absence of EBI2 from DCs resulted in defects in both the activation of CD4+T cells and the induction of antibody responses. Regulated expression of EBI2 on DC populations is therefore critical for the generation and correct positioning of splenic DCs and the initiation of immune responses.",

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AU - Gatto, Dominique

AU - Wood, Katherine

AU - Caminschi, Irina

AU - Murphy-Durland, Danielle

AU - Schofield, Peter

AU - Christ, Daniel

AU - Karupiah, Gunasegaran

AU - Brink, Robert

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AB - Spleen-resident dendritic cell (DC) populations occupy sentinel positions for the capture and presentation of blood-borne antigens. Here we found a difference in expression of the chemotactic receptor EBI2 (GPR183) on splenic DC subsets and that EBI2 regulated the positioning and homeostasis of DCs in the spleen. EBI2 and its main ligand, 7α,25-OHC, were required for the generation of the splenic CD4+DC subset and the localization of DCs in bridging channels. Absence of EBI2 from DCs resulted in defects in both the activation of CD4+T cells and the induction of antibody responses. Regulated expression of EBI2 on DC populations is therefore critical for the generation and correct positioning of splenic DCs and the initiation of immune responses.

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The chemotactic receptor EBI2 regulates the homeostasis, localization and immunological function of splenic dendritic cells

Abstract

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