The contribution of plastidial phosphoglucomutase to the control of starch synthesis within the potato tuber

A. R. Fernie; Ute Roessner; Richard N Trethewey; Lothar Willmitzer

doi:10.1007/s004250100521

The contribution of plastidial phosphoglucomutase to the control of starch synthesis within the potato tuber

A. R. Fernie, Ute Roessner, Richard N Trethewey, Lothar Willmitzer

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73 Citations (Scopus)

Abstract

The aim of this work was to evaluate the extent to which plastidial phosphoglucomutase (PGM) activity controls starch synthesis within potato (Solanum tuberosum L. cv. Desirée) tubers. The reduction in the activity of plastidial PGM led to both a correlative reduction in starch accumulation and an increased sucrose accumulation. The control coefficient of plastidial PGM on the accumulation of starch was estimated to approximate 0.24. The fluxes of carbohydrate metabolism were measured by investigating the metabolism of [U-¹⁴C]glucose in tuber discs from wild-type and transgenic plants. In tuber discs the control coefficient of plastidial PGM over starch synthesis was estimated as 0.36, indicating that this enzyme exerts considerable control over starch synthesis within the potato tuber.

Original language	English
Pages (from-to)	418-426
Number of pages	9
Journal	Planta
Volume	213
Issue number	3
DOIs	https://doi.org/10.1007/s004250100521
Publication status	Published - 2001
Externally published	Yes

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title = "The contribution of plastidial phosphoglucomutase to the control of starch synthesis within the potato tuber",

abstract = "The aim of this work was to evaluate the extent to which plastidial phosphoglucomutase (PGM) activity controls starch synthesis within potato (Solanum tuberosum L. cv. Desir{\'e}e) tubers. The reduction in the activity of plastidial PGM led to both a correlative reduction in starch accumulation and an increased sucrose accumulation. The control coefficient of plastidial PGM on the accumulation of starch was estimated to approximate 0.24. The fluxes of carbohydrate metabolism were measured by investigating the metabolism of [U-14C]glucose in tuber discs from wild-type and transgenic plants. In tuber discs the control coefficient of plastidial PGM over starch synthesis was estimated as 0.36, indicating that this enzyme exerts considerable control over starch synthesis within the potato tuber.",

keywords = "Metabolic control analysis, Phosphoglucomutase (plastidial), Solanum tuberosum, Starch synthesis, Sucrose (re)synthesis",

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T1 - The contribution of plastidial phosphoglucomutase to the control of starch synthesis within the potato tuber

AU - Fernie, A. R.

AU - Roessner, Ute

AU - Trethewey, Richard N

AU - Willmitzer, Lothar

PY - 2001

Y1 - 2001

N2 - The aim of this work was to evaluate the extent to which plastidial phosphoglucomutase (PGM) activity controls starch synthesis within potato (Solanum tuberosum L. cv. Desirée) tubers. The reduction in the activity of plastidial PGM led to both a correlative reduction in starch accumulation and an increased sucrose accumulation. The control coefficient of plastidial PGM on the accumulation of starch was estimated to approximate 0.24. The fluxes of carbohydrate metabolism were measured by investigating the metabolism of [U-14C]glucose in tuber discs from wild-type and transgenic plants. In tuber discs the control coefficient of plastidial PGM over starch synthesis was estimated as 0.36, indicating that this enzyme exerts considerable control over starch synthesis within the potato tuber.

AB - The aim of this work was to evaluate the extent to which plastidial phosphoglucomutase (PGM) activity controls starch synthesis within potato (Solanum tuberosum L. cv. Desirée) tubers. The reduction in the activity of plastidial PGM led to both a correlative reduction in starch accumulation and an increased sucrose accumulation. The control coefficient of plastidial PGM on the accumulation of starch was estimated to approximate 0.24. The fluxes of carbohydrate metabolism were measured by investigating the metabolism of [U-14C]glucose in tuber discs from wild-type and transgenic plants. In tuber discs the control coefficient of plastidial PGM over starch synthesis was estimated as 0.36, indicating that this enzyme exerts considerable control over starch synthesis within the potato tuber.

KW - Metabolic control analysis

KW - Phosphoglucomutase (plastidial)

KW - Solanum tuberosum

KW - Starch synthesis

KW - Sucrose (re)synthesis

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The contribution of plastidial phosphoglucomutase to the control of starch synthesis within the potato tuber

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