The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1

Siew Siew Pang; Charles Bayly-Jones; Mazdak Radjainia; Bradley A. Spicer; Ruby H.P. Law; Adrian W. Hodel; Edward S. Parsons; Susan M. Ekkel; Paul J. Conroy; Georg Ramm; Hariprasad Venugopal; Phillip I. Bird; Bart W. Hoogenboom; Ilia Voskoboinik; Yann Gambin; Emma Sierecki; Michelle A. Dunstone; James C. Whisstock

doi:10.1038/s41467-019-12279-2

The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1

Siew Siew Pang, Charles Bayly-Jones, Mazdak Radjainia, Bradley A. Spicer, Ruby H.P. Law, Adrian W. Hodel, Edward S. Parsons, Susan M. Ekkel, Paul J. Conroy, Georg Ramm, Hariprasad Venugopal, Phillip I. Bird, Bart W. Hoogenboom, Ilia Voskoboinik, Yann Gambin, Emma Sierecki, Michelle A. Dunstone^*, James C. Whisstock

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

Macrophage-expressed gene 1 (MPEG1/Perforin-2) is a perforin-like protein that functions within the phagolysosome to damage engulfed microbes. MPEG1 is thought to form pores in target membranes, however, its mode of action remains unknown. We use cryo-Electron Microscopy (cryo-EM) to determine the 2.4 Å structure of a hexadecameric assembly of MPEG1 that displays the expected features of a soluble prepore complex. We further discover that MPEG1 prepore-like assemblies can be induced to perforate membranes through acidification, such as would occur within maturing phagolysosomes. We next solve the 3.6 Å cryo-EM structure of MPEG1 in complex with liposomes. These data reveal that a multi-vesicular body of 12 kDa (MVB12)-associated β-prism (MABP) domain binds membranes such that the pore-forming machinery of MPEG1 is oriented away from the bound membrane. This unexpected mechanism of membrane interaction suggests that MPEG1 remains bound to the phagolysosome membrane while simultaneously forming pores in engulfed bacterial targets.

Original language	English
Article number	4288
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-12279-2
Publication status	Published - 1 Dec 2019

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Pang, S. S., Bayly-Jones, C., Radjainia, M., Spicer, B. A., Law, R. H. P., Hodel, A. W., Parsons, E. S., Ekkel, S. M., Conroy, P. J., Ramm, G., Venugopal, H., Bird, P. I., Hoogenboom, B. W., Voskoboinik, I., Gambin, Y., Sierecki, E., Dunstone, M. A., & Whisstock, J. C. (2019). The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1. Nature Communications, 10(1), Article 4288. https://doi.org/10.1038/s41467-019-12279-2

@article{cc4a85d624ac4e7a960b9f0f6d9bb3af,

title = "The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1",

abstract = "Macrophage-expressed gene 1 (MPEG1/Perforin-2) is a perforin-like protein that functions within the phagolysosome to damage engulfed microbes. MPEG1 is thought to form pores in target membranes, however, its mode of action remains unknown. We use cryo-Electron Microscopy (cryo-EM) to determine the 2.4 {\AA} structure of a hexadecameric assembly of MPEG1 that displays the expected features of a soluble prepore complex. We further discover that MPEG1 prepore-like assemblies can be induced to perforate membranes through acidification, such as would occur within maturing phagolysosomes. We next solve the 3.6 {\AA} cryo-EM structure of MPEG1 in complex with liposomes. These data reveal that a multi-vesicular body of 12 kDa (MVB12)-associated β-prism (MABP) domain binds membranes such that the pore-forming machinery of MPEG1 is oriented away from the bound membrane. This unexpected mechanism of membrane interaction suggests that MPEG1 remains bound to the phagolysosome membrane while simultaneously forming pores in engulfed bacterial targets.",

author = "Pang, {Siew Siew} and Charles Bayly-Jones and Mazdak Radjainia and Spicer, {Bradley A.} and Law, {Ruby H.P.} and Hodel, {Adrian W.} and Parsons, {Edward S.} and Ekkel, {Susan M.} and Conroy, {Paul J.} and Georg Ramm and Hariprasad Venugopal and Bird, {Phillip I.} and Hoogenboom, {Bart W.} and Ilia Voskoboinik and Yann Gambin and Emma Sierecki and Dunstone, {Michelle A.} and Whisstock, {James C.}",

note = "Publisher Copyright: {\textcopyright} 2019, The Author(s).",

year = "2019",

month = dec,

day = "1",

doi = "10.1038/s41467-019-12279-2",

language = "English",

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Pang, SS, Bayly-Jones, C, Radjainia, M, Spicer, BA, Law, RHP, Hodel, AW, Parsons, ES, Ekkel, SM, Conroy, PJ, Ramm, G, Venugopal, H, Bird, PI, Hoogenboom, BW, Voskoboinik, I, Gambin, Y, Sierecki, E, Dunstone, MA & Whisstock, JC 2019, 'The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1', Nature Communications, vol. 10, no. 1, 4288. https://doi.org/10.1038/s41467-019-12279-2

TY - JOUR

T1 - The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1

AU - Pang, Siew Siew

AU - Bayly-Jones, Charles

AU - Radjainia, Mazdak

AU - Spicer, Bradley A.

AU - Law, Ruby H.P.

AU - Hodel, Adrian W.

AU - Parsons, Edward S.

AU - Ekkel, Susan M.

AU - Conroy, Paul J.

AU - Ramm, Georg

AU - Venugopal, Hariprasad

AU - Bird, Phillip I.

AU - Hoogenboom, Bart W.

AU - Voskoboinik, Ilia

AU - Gambin, Yann

AU - Sierecki, Emma

AU - Dunstone, Michelle A.

AU - Whisstock, James C.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Macrophage-expressed gene 1 (MPEG1/Perforin-2) is a perforin-like protein that functions within the phagolysosome to damage engulfed microbes. MPEG1 is thought to form pores in target membranes, however, its mode of action remains unknown. We use cryo-Electron Microscopy (cryo-EM) to determine the 2.4 Å structure of a hexadecameric assembly of MPEG1 that displays the expected features of a soluble prepore complex. We further discover that MPEG1 prepore-like assemblies can be induced to perforate membranes through acidification, such as would occur within maturing phagolysosomes. We next solve the 3.6 Å cryo-EM structure of MPEG1 in complex with liposomes. These data reveal that a multi-vesicular body of 12 kDa (MVB12)-associated β-prism (MABP) domain binds membranes such that the pore-forming machinery of MPEG1 is oriented away from the bound membrane. This unexpected mechanism of membrane interaction suggests that MPEG1 remains bound to the phagolysosome membrane while simultaneously forming pores in engulfed bacterial targets.

AB - Macrophage-expressed gene 1 (MPEG1/Perforin-2) is a perforin-like protein that functions within the phagolysosome to damage engulfed microbes. MPEG1 is thought to form pores in target membranes, however, its mode of action remains unknown. We use cryo-Electron Microscopy (cryo-EM) to determine the 2.4 Å structure of a hexadecameric assembly of MPEG1 that displays the expected features of a soluble prepore complex. We further discover that MPEG1 prepore-like assemblies can be induced to perforate membranes through acidification, such as would occur within maturing phagolysosomes. We next solve the 3.6 Å cryo-EM structure of MPEG1 in complex with liposomes. These data reveal that a multi-vesicular body of 12 kDa (MVB12)-associated β-prism (MABP) domain binds membranes such that the pore-forming machinery of MPEG1 is oriented away from the bound membrane. This unexpected mechanism of membrane interaction suggests that MPEG1 remains bound to the phagolysosome membrane while simultaneously forming pores in engulfed bacterial targets.

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U2 - 10.1038/s41467-019-12279-2

DO - 10.1038/s41467-019-12279-2

M3 - Article

SN - 2041-1723

VL - 10

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 4288

ER -

The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1

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