The effect of selenium nanoparticles on the osteogenic differentiation of MC3T3-E1 cells

Sang Cheol Lee; Na Hyun Lee; Kapil D. Patel; Tae Su Jang; Jonathan Campbell Knowles; Hae Won Kim; Hae Hyoung Lee; Jung Hwan Lee

doi:10.3390/nano11020557

The effect of selenium nanoparticles on the osteogenic differentiation of MC3T3-E1 cells

Sang Cheol Lee, Na Hyun Lee, Kapil D. Patel, Tae Su Jang, Jonathan Campbell Knowles, Hae Won Kim, Hae Hyoung Lee^*, Jung Hwan Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) regulate various functions of cells, including cell death, viability, and differentiation, and nanoparticles influence ROS depending on their size and shape. Selenium is known to regulate various physiological functions, such as cell differentiations and anti-inflammatory functions, and plays an important role in the regulation of ROS as an antioxidant. This study aims to investigate the effect of selenium nanoparticles (SeNPs) on the differentiation of osteogenic MC3T3-E1 cells. After fabrication of SeNPs with a size of 25.3 ± 2.6 nm, and confirmation of its oxidase-like activity, SeNPs were added to MC3T3-E1 cells with or without H2O2: 5~20 μg/mL SeNPs recovered cells damaged by 200 μM H2O2 via the intracellular ROS downregulating role of SeNPs, revealed by the ROS staining assay. The increase in osteogenic maturation with SeNPs was gradually investigated by expression of osteogenic genes at 3 and 7 days, Alkaline phosphatase activity staining at 14 days, and Alizarin red S staining at 28 days. Therefore, the role of SeNPs in regulating ROS and their therapeutic effects on the differentiation of MC3T3-E1 cells were deter-mined, leading to possible applications for bone treatment.

Original language	English
Article number	557
Pages (from-to)	1-15
Number of pages	15
Journal	Nanomaterials
Volume	11
Issue number	2
DOIs	https://doi.org/10.3390/nano11020557
Publication status	Published - Feb 2021
Externally published	Yes

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title = "The effect of selenium nanoparticles on the osteogenic differentiation of MC3T3-E1 cells",

abstract = "Reactive oxygen species (ROS) regulate various functions of cells, including cell death, viability, and differentiation, and nanoparticles influence ROS depending on their size and shape. Selenium is known to regulate various physiological functions, such as cell differentiations and anti-inflammatory functions, and plays an important role in the regulation of ROS as an antioxidant. This study aims to investigate the effect of selenium nanoparticles (SeNPs) on the differentiation of osteogenic MC3T3-E1 cells. After fabrication of SeNPs with a size of 25.3 ± 2.6 nm, and confirmation of its oxidase-like activity, SeNPs were added to MC3T3-E1 cells with or without H2O2: 5~20 μg/mL SeNPs recovered cells damaged by 200 μM H2O2 via the intracellular ROS downregulating role of SeNPs, revealed by the ROS staining assay. The increase in osteogenic maturation with SeNPs was gradually investigated by expression of osteogenic genes at 3 and 7 days, Alkaline phosphatase activity staining at 14 days, and Alizarin red S staining at 28 days. Therefore, the role of SeNPs in regulating ROS and their therapeutic effects on the differentiation of MC3T3-E1 cells were deter-mined, leading to possible applications for bone treatment.",

keywords = "MC3T3-E1, Nanoparticles, Osteogenic differentiation, Reactive oxygen species, Selenium",

author = "Lee, {Sang Cheol} and Lee, {Na Hyun} and Patel, {Kapil D.} and Jang, {Tae Su} and Knowles, {Jonathan Campbell} and Kim, {Hae Won} and Lee, {Hae Hyoung} and Lee, {Jung Hwan}",

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year = "2021",

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volume = "11",

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T1 - The effect of selenium nanoparticles on the osteogenic differentiation of MC3T3-E1 cells

AU - Lee, Sang Cheol

AU - Lee, Na Hyun

AU - Patel, Kapil D.

AU - Jang, Tae Su

AU - Knowles, Jonathan Campbell

AU - Kim, Hae Won

AU - Lee, Hae Hyoung

AU - Lee, Jung Hwan

PY - 2021/2

Y1 - 2021/2

N2 - Reactive oxygen species (ROS) regulate various functions of cells, including cell death, viability, and differentiation, and nanoparticles influence ROS depending on their size and shape. Selenium is known to regulate various physiological functions, such as cell differentiations and anti-inflammatory functions, and plays an important role in the regulation of ROS as an antioxidant. This study aims to investigate the effect of selenium nanoparticles (SeNPs) on the differentiation of osteogenic MC3T3-E1 cells. After fabrication of SeNPs with a size of 25.3 ± 2.6 nm, and confirmation of its oxidase-like activity, SeNPs were added to MC3T3-E1 cells with or without H2O2: 5~20 μg/mL SeNPs recovered cells damaged by 200 μM H2O2 via the intracellular ROS downregulating role of SeNPs, revealed by the ROS staining assay. The increase in osteogenic maturation with SeNPs was gradually investigated by expression of osteogenic genes at 3 and 7 days, Alkaline phosphatase activity staining at 14 days, and Alizarin red S staining at 28 days. Therefore, the role of SeNPs in regulating ROS and their therapeutic effects on the differentiation of MC3T3-E1 cells were deter-mined, leading to possible applications for bone treatment.

AB - Reactive oxygen species (ROS) regulate various functions of cells, including cell death, viability, and differentiation, and nanoparticles influence ROS depending on their size and shape. Selenium is known to regulate various physiological functions, such as cell differentiations and anti-inflammatory functions, and plays an important role in the regulation of ROS as an antioxidant. This study aims to investigate the effect of selenium nanoparticles (SeNPs) on the differentiation of osteogenic MC3T3-E1 cells. After fabrication of SeNPs with a size of 25.3 ± 2.6 nm, and confirmation of its oxidase-like activity, SeNPs were added to MC3T3-E1 cells with or without H2O2: 5~20 μg/mL SeNPs recovered cells damaged by 200 μM H2O2 via the intracellular ROS downregulating role of SeNPs, revealed by the ROS staining assay. The increase in osteogenic maturation with SeNPs was gradually investigated by expression of osteogenic genes at 3 and 7 days, Alkaline phosphatase activity staining at 14 days, and Alizarin red S staining at 28 days. Therefore, the role of SeNPs in regulating ROS and their therapeutic effects on the differentiation of MC3T3-E1 cells were deter-mined, leading to possible applications for bone treatment.

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The effect of selenium nanoparticles on the osteogenic differentiation of MC3T3-E1 cells

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