TY - JOUR
T1 - The Expanding Class 2 CRISPR Toolbox
T2 - Diversity, Applicability, and Targeting Drawbacks
AU - Hajizadeh Dastjerdi, Arash
AU - Newman, Anthony
AU - Burgio, Gaetan
N1 - Publisher Copyright:
© 2019, Springer Nature Switzerland AG.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - The class 2 clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system, one of the prokaryotic adaptive immune systems, has sparked a lot of attention for its use as a gene editing tool. Currently, type II, V, and VI effector modules of this class have been characterized and extensively tested for nucleic acid editing, imaging, and disease diagnostics. Due to the unique composition of their nuclease catalytic center, the effector modules substantially vary in their function and possible biotechnology applications. In this review, we discuss the structural and functional diversity in class 2 CRISPR effectors, and debate their suitability for nucleic acid targeting and their shortcomings as gene editing tools.
AB - The class 2 clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system, one of the prokaryotic adaptive immune systems, has sparked a lot of attention for its use as a gene editing tool. Currently, type II, V, and VI effector modules of this class have been characterized and extensively tested for nucleic acid editing, imaging, and disease diagnostics. Due to the unique composition of their nuclease catalytic center, the effector modules substantially vary in their function and possible biotechnology applications. In this review, we discuss the structural and functional diversity in class 2 CRISPR effectors, and debate their suitability for nucleic acid targeting and their shortcomings as gene editing tools.
UR - http://www.scopus.com/inward/record.url?scp=85070217014&partnerID=8YFLogxK
U2 - 10.1007/s40259-019-00369-y
DO - 10.1007/s40259-019-00369-y
M3 - Review article
SN - 1173-8804
VL - 33
SP - 503
EP - 513
JO - BioDrugs
JF - BioDrugs
IS - 5
ER -