The Expanding Class 2 CRISPR Toolbox: Diversity, Applicability, and Targeting Drawbacks

Arash Hajizadeh Dastjerdi; Anthony Newman; Gaetan Burgio

doi:10.1007/s40259-019-00369-y

The Expanding Class 2 CRISPR Toolbox: Diversity, Applicability, and Targeting Drawbacks

Arash Hajizadeh Dastjerdi, Anthony Newman, Gaetan Burgio^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

14 Citations (Scopus)

Abstract

The class 2 clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system, one of the prokaryotic adaptive immune systems, has sparked a lot of attention for its use as a gene editing tool. Currently, type II, V, and VI effector modules of this class have been characterized and extensively tested for nucleic acid editing, imaging, and disease diagnostics. Due to the unique composition of their nuclease catalytic center, the effector modules substantially vary in their function and possible biotechnology applications. In this review, we discuss the structural and functional diversity in class 2 CRISPR effectors, and debate their suitability for nucleic acid targeting and their shortcomings as gene editing tools.

Original language	English
Pages (from-to)	503-513
Number of pages	11
Journal	BioDrugs
Volume	33
Issue number	5
DOIs	https://doi.org/10.1007/s40259-019-00369-y
Publication status	Published - 1 Oct 2019

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title = "The Expanding Class 2 CRISPR Toolbox: Diversity, Applicability, and Targeting Drawbacks",

abstract = "The class 2 clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system, one of the prokaryotic adaptive immune systems, has sparked a lot of attention for its use as a gene editing tool. Currently, type II, V, and VI effector modules of this class have been characterized and extensively tested for nucleic acid editing, imaging, and disease diagnostics. Due to the unique composition of their nuclease catalytic center, the effector modules substantially vary in their function and possible biotechnology applications. In this review, we discuss the structural and functional diversity in class 2 CRISPR effectors, and debate their suitability for nucleic acid targeting and their shortcomings as gene editing tools.",

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AU - Burgio, Gaetan

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N2 - The class 2 clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system, one of the prokaryotic adaptive immune systems, has sparked a lot of attention for its use as a gene editing tool. Currently, type II, V, and VI effector modules of this class have been characterized and extensively tested for nucleic acid editing, imaging, and disease diagnostics. Due to the unique composition of their nuclease catalytic center, the effector modules substantially vary in their function and possible biotechnology applications. In this review, we discuss the structural and functional diversity in class 2 CRISPR effectors, and debate their suitability for nucleic acid targeting and their shortcomings as gene editing tools.

AB - The class 2 clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system, one of the prokaryotic adaptive immune systems, has sparked a lot of attention for its use as a gene editing tool. Currently, type II, V, and VI effector modules of this class have been characterized and extensively tested for nucleic acid editing, imaging, and disease diagnostics. Due to the unique composition of their nuclease catalytic center, the effector modules substantially vary in their function and possible biotechnology applications. In this review, we discuss the structural and functional diversity in class 2 CRISPR effectors, and debate their suitability for nucleic acid targeting and their shortcomings as gene editing tools.

UR - https://www.scopus.com/pages/publications/85070217014

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DO - 10.1007/s40259-019-00369-y

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JF - BioDrugs

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ER -

The Expanding Class 2 CRISPR Toolbox: Diversity, Applicability, and Targeting Drawbacks

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