The impact of glutathione transferase kappa deficiency on adiponectin multimerisation in vivo

Angelo Theodoratos, Anneke Blackburn, Marjorie Coggan, Jean Cappello, Claire Larter, Klaus Matthaei, Philip Board

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    Glutathione transferase Kappa (GSTK1-1) also termed disulfide bond-forming oxidoreductase A-like protein (DsbA-L) has been implicated in the post-translational multimerization of adiponectin and has been negatively correlated with obesity in mice and humans. We investigated adiponectin in Gstk1-/- mice and surprisingly found no difference in the levels of total serum adiponectin or the level of high molecular weight (HMW) multimers when compared with normal controls. Non-reducing SDS-polyacrylamide gel electrophoresis and western blotting also showed a similar distribution of low, middle and HMW multimers in normal and Gstk1-/- mice. Variation in adiponectin has been correlated with glucose tolerance and with the levels of phosphorylated AMP-kinase but we found similar glucose tolerance and similar levels of phospho 5-AMP-activated protein kinase in normal and Gstk1-/- mice. Consequently, our findings suggest that GSTK1-1 is not absolutely required for adiponectin multimerization in vivo and alternate pathways may be activated in GSTK1-1 deficiency.International Journal of Obesity advance online publication, 17 January 2012; doi:10.1038/ijo.2011.267.
    Original languageEnglish
    Pages (from-to)1366-1369
    JournalInternational Journal of Obesity
    Issue number10
    Publication statusPublished - 2012


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