The merozoite-specific protein, TgGRA11B, identified as a component of the Toxoplasma gondii parasitophorous vacuole in a tachyzoite expression model

Chandra Ramakrishnan; Robert A. Walker; Ramon M. Eichenberger; Adrian B. Hehl; Nicholas C. Smith

doi:10.1016/j.ijpara.2017.04.001

The merozoite-specific protein, TgGRA11B, identified as a component of the Toxoplasma gondii parasitophorous vacuole in a tachyzoite expression model

Chandra Ramakrishnan^*, Robert A. Walker, Ramon M. Eichenberger, Adrian B. Hehl, Nicholas C. Smith

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The apicomplexan, Toxoplasma gondii, infects all warm-blooded animals as intermediate hosts but only felids as definitive hosts. Dense granule proteins are critical for the survival of Toxoplasma within host cells but, whilst these proteins have been studied intensively in tachyzoites, little is known about their expression in the coccidian stages in the cat intestine. Transcriptomic profiling indicates that two putative dense granule proteins, TgGRA11A and TgGRA11B, are expressed uniquely in merozoites. Immunofluorescent microscopy of Toxoplasma-infected cat intestine and tachyzoites engineered to express TgGRA11B, reveals that it is a dense granule protein that traffics into the parasitophorous vacuole and its membrane.

Original language	English
Pages (from-to)	597-600
Number of pages	4
Journal	International Journal for Parasitology
Volume	47
Issue number	10-11
DOIs	https://doi.org/10.1016/j.ijpara.2017.04.001
Publication status	Published - Sept 2017

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10.1016/j.ijpara.2017.04.001

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title = "The merozoite-specific protein, TgGRA11B, identified as a component of the Toxoplasma gondii parasitophorous vacuole in a tachyzoite expression model",

abstract = "The apicomplexan, Toxoplasma gondii, infects all warm-blooded animals as intermediate hosts but only felids as definitive hosts. Dense granule proteins are critical for the survival of Toxoplasma within host cells but, whilst these proteins have been studied intensively in tachyzoites, little is known about their expression in the coccidian stages in the cat intestine. Transcriptomic profiling indicates that two putative dense granule proteins, TgGRA11A and TgGRA11B, are expressed uniquely in merozoites. Immunofluorescent microscopy of Toxoplasma-infected cat intestine and tachyzoites engineered to express TgGRA11B, reveals that it is a dense granule protein that traffics into the parasitophorous vacuole and its membrane.",

keywords = "Coccidia, Dense granule, Merozoite, Parasitophorous vacuole, TgGRA11B, Toxoplasma gondii",

author = "Chandra Ramakrishnan and Walker, {Robert A.} and Eichenberger, {Ramon M.} and Hehl, {Adrian B.} and Smith, {Nicholas C.}",

note = "Publisher Copyright: {\textcopyright} 2017 Australian Society for Parasitology",

year = "2017",

month = sep,

doi = "10.1016/j.ijpara.2017.04.001",

language = "English",

volume = "47",

pages = "597--600",

journal = "International Journal for Parasitology",

issn = "0020-7519",

publisher = "Elsevier Ltd.",

number = "10-11",

}

The merozoite-specific protein, TgGRA11B, identified as a component of the Toxoplasma gondii parasitophorous vacuole in a tachyzoite expression model. / Ramakrishnan, Chandra; Walker, Robert A.; Eichenberger, Ramon M. et al.
In: International Journal for Parasitology, Vol. 47, No. 10-11, 09.2017, p. 597-600.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The merozoite-specific protein, TgGRA11B, identified as a component of the Toxoplasma gondii parasitophorous vacuole in a tachyzoite expression model

AU - Ramakrishnan, Chandra

AU - Walker, Robert A.

AU - Eichenberger, Ramon M.

AU - Hehl, Adrian B.

AU - Smith, Nicholas C.

PY - 2017/9

Y1 - 2017/9

N2 - The apicomplexan, Toxoplasma gondii, infects all warm-blooded animals as intermediate hosts but only felids as definitive hosts. Dense granule proteins are critical for the survival of Toxoplasma within host cells but, whilst these proteins have been studied intensively in tachyzoites, little is known about their expression in the coccidian stages in the cat intestine. Transcriptomic profiling indicates that two putative dense granule proteins, TgGRA11A and TgGRA11B, are expressed uniquely in merozoites. Immunofluorescent microscopy of Toxoplasma-infected cat intestine and tachyzoites engineered to express TgGRA11B, reveals that it is a dense granule protein that traffics into the parasitophorous vacuole and its membrane.

AB - The apicomplexan, Toxoplasma gondii, infects all warm-blooded animals as intermediate hosts but only felids as definitive hosts. Dense granule proteins are critical for the survival of Toxoplasma within host cells but, whilst these proteins have been studied intensively in tachyzoites, little is known about their expression in the coccidian stages in the cat intestine. Transcriptomic profiling indicates that two putative dense granule proteins, TgGRA11A and TgGRA11B, are expressed uniquely in merozoites. Immunofluorescent microscopy of Toxoplasma-infected cat intestine and tachyzoites engineered to express TgGRA11B, reveals that it is a dense granule protein that traffics into the parasitophorous vacuole and its membrane.

KW - Coccidia

KW - Dense granule

KW - Merozoite

KW - Parasitophorous vacuole

KW - TgGRA11B

KW - Toxoplasma gondii

UR - http://www.scopus.com/inward/record.url?scp=85019558320&partnerID=8YFLogxK

U2 - 10.1016/j.ijpara.2017.04.001

DO - 10.1016/j.ijpara.2017.04.001

M3 - Article

SN - 0020-7519

VL - 47

SP - 597

EP - 600

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 10-11

ER -

The merozoite-specific protein, TgGRA11B, identified as a component of the Toxoplasma gondii parasitophorous vacuole in a tachyzoite expression model

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