The Rhizobium strain Ngr234 Nod DA gene product responds to activation by the simple phenolic compounds vanillin and isovanillin present in wheat seedling extracts

Kathryn K. Le Strange; Gregory L. Bender; Michael A. Djordjevic; Barry G. Rolfe; John W. Redmond

doi:10.1094/MPMI-3-214

The Rhizobium strain Ngr234 Nod DA gene product responds to activation by the simple phenolic compounds vanillin and isovanillin present in wheat seedling extracts

Kathryn K. Le Strange, Gregory L. Bender, Michael A. Djordjevic, Barry G. Rolfe^*, John W. Redmond

^*Corresponding author for this work

Research School of Biology

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65 Citations (Scopus)

Abstract

Vanillin and isovanillin are present in extracts of wheat seedlings and interact with the nodulation (nod) gene nodDl from Rhizobium strain NGR234 to induce expression of R, leguminosarum bv. trifolii nod genes. Seven varieties of Australian wheat were examined. Vanillin, isovanillin, or both were present in five of the varieties tested. Assays of a wide range of authentic flavonoid and other phenolic compounds for transcriptional induction of the same nodAlacZ fusion revealed that a hydroxyl group para to an electron-withdrawing group and/or the presence of a cluster of oxygen functions are the prime structural requisites for transcriptional activation of NodDl-activated nod genes.

Original language	English
Pages (from-to)	214-220
Number of pages	7
Journal	Molecular Plant-Microbe Interactions
Volume	3
Issue number	4
DOIs	https://doi.org/10.1094/MPMI-3-214
Publication status	Published - 1990

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title = "The Rhizobium strain Ngr234 Nod DA gene product responds to activation by the simple phenolic compounds vanillin and isovanillin present in wheat seedling extracts",

abstract = "Vanillin and isovanillin are present in extracts of wheat seedlings and interact with the nodulation (nod) gene nodDl from Rhizobium strain NGR234 to induce expression of R, leguminosarum bv. trifolii nod genes. Seven varieties of Australian wheat were examined. Vanillin, isovanillin, or both were present in five of the varieties tested. Assays of a wide range of authentic flavonoid and other phenolic compounds for transcriptional induction of the same nodAlacZ fusion revealed that a hydroxyl group para to an electron-withdrawing group and/or the presence of a cluster of oxygen functions are the prime structural requisites for transcriptional activation of NodDl-activated nod genes.",

keywords = "Nod gene induction, NodD, Phenolic inducers, Wheat extract",

author = "{Le Strange}, {Kathryn K.} and Bender, {Gregory L.} and Djordjevic, {Michael A.} and Rolfe, {Barry G.} and Redmond, {John W.}",

note = "Publisher Copyright: {\textcopyright} 1990 The American Phytopathological Society",

year = "1990",

doi = "10.1094/MPMI-3-214",

language = "English",

volume = "3",

pages = "214--220",

journal = "Molecular Plant-Microbe Interactions",

issn = "0894-0282",

publisher = "American Phytopathological Society",

number = "4",

}

The Rhizobium strain Ngr234 Nod DA gene product responds to activation by the simple phenolic compounds vanillin and isovanillin present in wheat seedling extracts. / Le Strange, Kathryn K.; Bender, Gregory L.; Djordjevic, Michael A. et al.
In: Molecular Plant-Microbe Interactions, Vol. 3, No. 4, 1990, p. 214-220.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The Rhizobium strain Ngr234 Nod DA gene product responds to activation by the simple phenolic compounds vanillin and isovanillin present in wheat seedling extracts

AU - Le Strange, Kathryn K.

AU - Bender, Gregory L.

AU - Djordjevic, Michael A.

AU - Rolfe, Barry G.

AU - Redmond, John W.

PY - 1990

Y1 - 1990

N2 - Vanillin and isovanillin are present in extracts of wheat seedlings and interact with the nodulation (nod) gene nodDl from Rhizobium strain NGR234 to induce expression of R, leguminosarum bv. trifolii nod genes. Seven varieties of Australian wheat were examined. Vanillin, isovanillin, or both were present in five of the varieties tested. Assays of a wide range of authentic flavonoid and other phenolic compounds for transcriptional induction of the same nodAlacZ fusion revealed that a hydroxyl group para to an electron-withdrawing group and/or the presence of a cluster of oxygen functions are the prime structural requisites for transcriptional activation of NodDl-activated nod genes.

AB - Vanillin and isovanillin are present in extracts of wheat seedlings and interact with the nodulation (nod) gene nodDl from Rhizobium strain NGR234 to induce expression of R, leguminosarum bv. trifolii nod genes. Seven varieties of Australian wheat were examined. Vanillin, isovanillin, or both were present in five of the varieties tested. Assays of a wide range of authentic flavonoid and other phenolic compounds for transcriptional induction of the same nodAlacZ fusion revealed that a hydroxyl group para to an electron-withdrawing group and/or the presence of a cluster of oxygen functions are the prime structural requisites for transcriptional activation of NodDl-activated nod genes.

KW - Nod gene induction

KW - NodD

KW - Phenolic inducers

KW - Wheat extract

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U2 - 10.1094/MPMI-3-214

DO - 10.1094/MPMI-3-214

M3 - Article

AN - SCOPUS:84953461994

SN - 0894-0282

VL - 3

SP - 214

EP - 220

JO - Molecular Plant-Microbe Interactions

JF - Molecular Plant-Microbe Interactions

IS - 4

ER -

The Rhizobium strain Ngr234 Nod DA gene product responds to activation by the simple phenolic compounds vanillin and isovanillin present in wheat seedling extracts

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