The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels

Navid Bavi, D. Marien Cortes, Charles D. Cox, Paul R. Rohde, Weihong Liu, Joachim W. Deitmer, Omid Bavi, Pavel Strop, Adam P. Hill, Douglas Rees, Ben Corry, Eduardo Perozo, Boris Martinac*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    75 Citations (Scopus)

    Abstract

    The bacterial mechanosensitive channel MscL gates in response to membrane tension as a result of mechanical force transmitted directly to the channel from the lipid bilayer. MscL represents an excellent model system to study the basic biophysical principles of mechanosensory transduction. However, understanding of the essential structural components that transduce bilayer tension into channel gating remains incomplete. Here using multiple experimental and computational approaches, we demonstrate that the amphipathic N-terminal helix of MscL acts as a crucial structural element during tension-induced gating, both stabilizing the closed state and coupling the channel to the membrane. We propose that this may also represent a common principle in the gating cycle of unrelated mechanosensitive ion channels, allowing the coupling of channel conformation to membrane dynamics.

    Original languageEnglish
    Article number11984
    JournalNature Communications
    Volume7
    DOIs
    Publication statusPublished - 22 Jun 2016

    Fingerprint

    Dive into the research topics of 'The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels'. Together they form a unique fingerprint.

    Cite this