The two forms of the S2 state multiline signal in Photosystem II: Effect of methanol and ethanol

K. A. Åhrling; M. C.W. Evans; J. H.A. Nugent; R. J. Pace

doi:10.1016/j.bbabio.2004.01.006

The two forms of the S₂ state multiline signal in Photosystem II: Effect of methanol and ethanol

K. A. Åhrling^*, M. C.W. Evans, J. H.A. Nugent, R. J. Pace

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The characteristic Mn hyperfine 'multiline' signal exhibited in the S ₂ state of the oxygen-evolving complex (OEC) complex of Photosystem II (PSII) has been shown to be heterogeneous in character. In this study, we have explored the effects that influence the proportions of the two forms of the S₂ state multiline signal present in any sample. The narrow form of the signal is lost upon storage (weeks) at 77 K, whereas the broad form remains. In particular, we explore the roles of ethanol and methanol as well as effects of the second turnover of the enzyme on storage of the sample at 77 K. We find that in samples containing methanol, the narrow form may predominate upon the first flash, but the broad form predominates on the fifth flash and also in samples containing ethanol.

Original language	English
Pages (from-to)	66-77
Number of pages	12
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	1656
Issue number	1
DOIs	https://doi.org/10.1016/j.bbabio.2004.01.006
Publication status	Published - 12 May 2004

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title = "The two forms of the S2 state multiline signal in Photosystem II: Effect of methanol and ethanol",

abstract = "The characteristic Mn hyperfine 'multiline' signal exhibited in the S 2 state of the oxygen-evolving complex (OEC) complex of Photosystem II (PSII) has been shown to be heterogeneous in character. In this study, we have explored the effects that influence the proportions of the two forms of the S2 state multiline signal present in any sample. The narrow form of the signal is lost upon storage (weeks) at 77 K, whereas the broad form remains. In particular, we explore the roles of ethanol and methanol as well as effects of the second turnover of the enzyme on storage of the sample at 77 K. We find that in samples containing methanol, the narrow form may predominate upon the first flash, but the broad form predominates on the fifth flash and also in samples containing ethanol.",

keywords = "ANU, Australian National University, Cyt, EPR, ESEEM, EtOH, Manganese, MeOH, Multiline signal, NIR, OEC, Photosystem II, cytochrome, electron paramagnetic resonance spectroscopy, electron spin echo envelope modulation, ethanol, methanol, near infrared",

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T1 - The two forms of the S2 state multiline signal in Photosystem II

T2 - Effect of methanol and ethanol

AU - Åhrling, K. A.

AU - Evans, M. C.W.

AU - Nugent, J. H.A.

AU - Pace, R. J.

PY - 2004/5/12

Y1 - 2004/5/12

N2 - The characteristic Mn hyperfine 'multiline' signal exhibited in the S 2 state of the oxygen-evolving complex (OEC) complex of Photosystem II (PSII) has been shown to be heterogeneous in character. In this study, we have explored the effects that influence the proportions of the two forms of the S2 state multiline signal present in any sample. The narrow form of the signal is lost upon storage (weeks) at 77 K, whereas the broad form remains. In particular, we explore the roles of ethanol and methanol as well as effects of the second turnover of the enzyme on storage of the sample at 77 K. We find that in samples containing methanol, the narrow form may predominate upon the first flash, but the broad form predominates on the fifth flash and also in samples containing ethanol.

AB - The characteristic Mn hyperfine 'multiline' signal exhibited in the S 2 state of the oxygen-evolving complex (OEC) complex of Photosystem II (PSII) has been shown to be heterogeneous in character. In this study, we have explored the effects that influence the proportions of the two forms of the S2 state multiline signal present in any sample. The narrow form of the signal is lost upon storage (weeks) at 77 K, whereas the broad form remains. In particular, we explore the roles of ethanol and methanol as well as effects of the second turnover of the enzyme on storage of the sample at 77 K. We find that in samples containing methanol, the narrow form may predominate upon the first flash, but the broad form predominates on the fifth flash and also in samples containing ethanol.

KW - ANU

KW - Australian National University

KW - Cyt

KW - EPR

KW - ESEEM

KW - EtOH

KW - Manganese

KW - MeOH

KW - Multiline signal

KW - NIR

KW - OEC

KW - Photosystem II

KW - cytochrome

KW - electron paramagnetic resonance spectroscopy

KW - electron spin echo envelope modulation

KW - ethanol

KW - methanol

KW - near infrared

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U2 - 10.1016/j.bbabio.2004.01.006

DO - 10.1016/j.bbabio.2004.01.006

M3 - Article

SN - 0005-2728

VL - 1656

SP - 66

EP - 77

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

IS - 1

ER -

The two forms of the S₂ state multiline signal in Photosystem II: Effect of methanol and ethanol

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