TY - JOUR
T1 - The whey acidic protein family
T2 - A new signature motif and three-dimensional structure by comparative modeling
AU - Ranganathan, Shoba
AU - Simpson, Kaylene J.
AU - Shaw, Denis C.
AU - Nicholas, Kevin R.
PY - 1999/4
Y1 - 1999/4
N2 - Whey acidic proteins (WAP) from the mouse, rat, rabbit, camel, and pig comprise two "fonr-disnlfide core" domains. From a detailed analysis of all sequences containing this domain, we propose a new PROSITE motif ([KRHGVLN]X-{PFj-X-[CF]-[PQSVLI]-X(9,19)-C-(P}-X-[DN]-X-/N}[CE]-X(5)-C-C) to accurately identify new fonr-disitlflde core proteins. A consensus model for the WAP proteins is proposed, based on the human mucous proteinase inhibitor crystal structure. This article presents a detailed atomic model for the two-domain porcine WAP sequence by comparative modeling. Surface electrostatic potential calculations indicate that the second domain of the pig WAP model is similar to the functional human mucous proteinase inhibitor domains, whereas the first domain may be nonfunctional.
AB - Whey acidic proteins (WAP) from the mouse, rat, rabbit, camel, and pig comprise two "fonr-disnlfide core" domains. From a detailed analysis of all sequences containing this domain, we propose a new PROSITE motif ([KRHGVLN]X-{PFj-X-[CF]-[PQSVLI]-X(9,19)-C-(P}-X-[DN]-X-/N}[CE]-X(5)-C-C) to accurately identify new fonr-disitlflde core proteins. A consensus model for the WAP proteins is proposed, based on the human mucous proteinase inhibitor crystal structure. This article presents a detailed atomic model for the two-domain porcine WAP sequence by comparative modeling. Surface electrostatic potential calculations indicate that the second domain of the pig WAP model is similar to the functional human mucous proteinase inhibitor domains, whereas the first domain may be nonfunctional.
KW - Four-disitlflde core
KW - Molecular electrostatic potential
KW - Molecular model
KW - Sequence motifs
KW - Sequence-structure comparison
KW - WAP
UR - http://www.scopus.com/inward/record.url?scp=0000056632&partnerID=8YFLogxK
U2 - 10.1016/S1093-3263(99)00023-6
DO - 10.1016/S1093-3263(99)00023-6
M3 - Article
SN - 1093-3263
VL - 17
SP - 106
EP - 113
JO - Journal of Molecular Graphics and Modelling
JF - Journal of Molecular Graphics and Modelling
IS - 2
ER -