Thiol-ene reaction: A versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR

Qing Feng Li; Yin Yang; Ansis Maleckis; Gottfried Otting; Xun Cheng Su

doi:10.1039/c2cc17900h

Thiol-ene reaction: A versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR

Qing Feng Li, Yin Yang, Ansis Maleckis, Gottfried Otting, Xun Cheng Su^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags.

Original language	English
Pages (from-to)	2704-2706
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	21
DOIs	https://doi.org/10.1039/c2cc17900h
Publication status	Published - 13 Feb 2012

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title = "Thiol-ene reaction: A versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR",

abstract = "Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags.",

author = "Li, {Qing Feng} and Yin Yang and Ansis Maleckis and Gottfried Otting and Su, {Xun Cheng}",

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AU - Li, Qing Feng

AU - Yang, Yin

AU - Maleckis, Ansis

AU - Otting, Gottfried

AU - Su, Xun Cheng

PY - 2012/2/13

Y1 - 2012/2/13

N2 - Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags.

AB - Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags.

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U2 - 10.1039/c2cc17900h

DO - 10.1039/c2cc17900h

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SP - 2704

EP - 2706

JO - Chemical Communications

JF - Chemical Communications

IS - 21

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Thiol-ene reaction: A versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR

Abstract

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