TY - GEN
T1 - Three-dimensional multi-site two-photon excitation for probing neuronal signal integration
AU - Go, M. A.
AU - Stricker, C.
AU - Redman, S.
AU - Bachor, H. A.
AU - Daria, V. R.
PY - 2011
Y1 - 2011
N2 - Two-photon holographic microscopy (2PHM) offers the advantage of simultaneous multi-site excitation in three dimensions. This is useful for studies of neuronal signal integration, which require multiple controlled synaptic inputs delivered simultaneously onto dendritic trees. In this work, we holographically split a single femtosecond pulse-laser in order to project multiple foci onto the neuron. At each focus, two-photon photolysis of caged neurotransmitter molecules, which bind to receptors and mimic synaptic transmission, is performed, thereby allowing measurement of how neurons integrate multiple synaptic inputs.
AB - Two-photon holographic microscopy (2PHM) offers the advantage of simultaneous multi-site excitation in three dimensions. This is useful for studies of neuronal signal integration, which require multiple controlled synaptic inputs delivered simultaneously onto dendritic trees. In this work, we holographically split a single femtosecond pulse-laser in order to project multiple foci onto the neuron. At each focus, two-photon photolysis of caged neurotransmitter molecules, which bind to receptors and mimic synaptic transmission, is performed, thereby allowing measurement of how neurons integrate multiple synaptic inputs.
UR - http://www.scopus.com/inward/record.url?scp=84893575088&partnerID=8YFLogxK
M3 - Conference contribution
SN - 9780977565771
T3 - Optics InfoBase Conference Papers
SP - 763
EP - 765
BT - Conference on Lasers and Electro-Optics/Pacific Rim, CLEOPR 2011
T2 - Conference on Lasers and Electro-Optics/Pacific Rim, CLEOPR 2011
Y2 - 28 August 2011 through 1 September 2011
ER -