Time-resolved Laue studies from dienelactone hydrolase and porphobilinogen deaminase

Paul D Carr; Anna Robinson; David Ollis; A Hadener; A C Niemann; J R Helliwell; J Habash; A Cassetta; T Ursby; D Bourgois; F Schotte; M Wulff

Time-resolved Laue studies from dienelactone hydrolase and porphobilinogen deaminase

Paul D Carr, Anna Robinson, David Ollis, A Hadener, A C Niemann, J R Helliwell, J Habash, A Cassetta, T Ursby, D Bourgois, F Schotte, M Wulff

Research output: Contribution to journal › Literature review

Abstract

Flow cell experiments were performed on both crystal systems using substrates of varying efficacy. Time-dependent intensity changes were observed during the PD experiments that are in broad agreement with solution kinetic measurements (Neimann et al., 1994). Mutant C123S crystals of DLH were used for the flow cell experiments because previous work (Pathak & Ollis, 1990) has shown that wild type crystals are susceptible to deactivation due to oxidation of the active site residue Cys123. Disordering/reordering phenomena were observed when the substrates dienelactone and methyl dienelactone were flowed over these crystals. In addition to the flow cell experiments data were collected on wild type DLH crystals to study the time-dependent nature of the oxidation of the active site Cys123 residue. Data were collected both on the in house CCD detector system and image plates. Initial results will be presented from these two studies

Original language	English
Pages (from-to)	c49-c50
Journal	Acta Crystallographica. Section A
Volume	52
Issue number	a1
Publication status	Published - 1996

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@article{9e06fd24df0a4901accbb28ce68af8de,

title = "Time-resolved Laue studies from dienelactone hydrolase and porphobilinogen deaminase",

abstract = "Flow cell experiments were performed on both crystal systems using substrates of varying efficacy. Time-dependent intensity changes were observed during the PD experiments that are in broad agreement with solution kinetic measurements (Neimann et al., 1994). Mutant C123S crystals of DLH were used for the flow cell experiments because previous work (Pathak & Ollis, 1990) has shown that wild type crystals are susceptible to deactivation due to oxidation of the active site residue Cys123. Disordering/reordering phenomena were observed when the substrates dienelactone and methyl dienelactone were flowed over these crystals. In addition to the flow cell experiments data were collected on wild type DLH crystals to study the time-dependent nature of the oxidation of the active site Cys123 residue. Data were collected both on the in house CCD detector system and image plates. Initial results will be presented from these two studies",

author = "Carr, {Paul D} and Anna Robinson and David Ollis and A Hadener and Niemann, {A C} and Helliwell, {J R} and J Habash and A Cassetta and T Ursby and D Bourgois and F Schotte and M Wulff",

year = "1996",

language = "English",

volume = "52",

pages = "c49--c50",

journal = "Acta Crystallographica. Section A ",

issn = "2053-2733",

publisher = "John Wiley & Sons Inc.",

number = "a1",

}

TY - JOUR

T1 - Time-resolved Laue studies from dienelactone hydrolase and porphobilinogen deaminase

AU - Carr, Paul D

AU - Robinson, Anna

AU - Ollis, David

AU - Hadener, A

AU - Niemann, A C

AU - Helliwell, J R

AU - Habash, J

AU - Cassetta, A

AU - Ursby, T

AU - Bourgois, D

AU - Schotte, F

AU - Wulff, M

PY - 1996

Y1 - 1996

N2 - Flow cell experiments were performed on both crystal systems using substrates of varying efficacy. Time-dependent intensity changes were observed during the PD experiments that are in broad agreement with solution kinetic measurements (Neimann et al., 1994). Mutant C123S crystals of DLH were used for the flow cell experiments because previous work (Pathak & Ollis, 1990) has shown that wild type crystals are susceptible to deactivation due to oxidation of the active site residue Cys123. Disordering/reordering phenomena were observed when the substrates dienelactone and methyl dienelactone were flowed over these crystals. In addition to the flow cell experiments data were collected on wild type DLH crystals to study the time-dependent nature of the oxidation of the active site Cys123 residue. Data were collected both on the in house CCD detector system and image plates. Initial results will be presented from these two studies

AB - Flow cell experiments were performed on both crystal systems using substrates of varying efficacy. Time-dependent intensity changes were observed during the PD experiments that are in broad agreement with solution kinetic measurements (Neimann et al., 1994). Mutant C123S crystals of DLH were used for the flow cell experiments because previous work (Pathak & Ollis, 1990) has shown that wild type crystals are susceptible to deactivation due to oxidation of the active site residue Cys123. Disordering/reordering phenomena were observed when the substrates dienelactone and methyl dienelactone were flowed over these crystals. In addition to the flow cell experiments data were collected on wild type DLH crystals to study the time-dependent nature of the oxidation of the active site Cys123 residue. Data were collected both on the in house CCD detector system and image plates. Initial results will be presented from these two studies

M3 - Literature review

SN - 2053-2733

VL - 52

SP - c49-c50

JO - Acta Crystallographica. Section A

JF - Acta Crystallographica. Section A

IS - a1

ER -

Time-resolved Laue studies from dienelactone hydrolase and porphobilinogen deaminase

Abstract

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