Topology and identification of critical residues of the O-acetyltransferase of serotype-converting bacteriophage, SF6, of Shigella flexneri

Farzaana Thanweer; Vikas Tahiliani; Haralambos Korres; Naresh K. Verma

doi:10.1016/j.bbrc.2008.08.069

Topology and identification of critical residues of the O-acetyltransferase of serotype-converting bacteriophage, SF6, of Shigella flexneri

Farzaana Thanweer, Vikas Tahiliani, Haralambos Korres, Naresh K. Verma^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (SciVal)

Abstract

The modification of the LPS O-antigen, seen in the diverse serotypes of Shigella flexneri is brought about by the glucosyltransferases (Gtr) and the O-acetyltransferase (Oac). In this study, we establish the membrane topology of Oac using the dual reporter PhoA-LacZα. We have determined that Oac is an integral membrane protein with 10 transmembrane regions. The hydrophilic N- and C-termini are oriented in the cytoplasm. Functionally important cytoplasmic and periplasmic loops have also been identified. Furthermore, cytoplasmic residues R73 and R75R76 were found to be critical to Oac function.

Original language	English
Pages (from-to)	581-585
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	375
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2008.08.069
Publication status	Published - 31 Oct 2008

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10.1016/j.bbrc.2008.08.069

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title = "Topology and identification of critical residues of the O-acetyltransferase of serotype-converting bacteriophage, SF6, of Shigella flexneri",

abstract = "The modification of the LPS O-antigen, seen in the diverse serotypes of Shigella flexneri is brought about by the glucosyltransferases (Gtr) and the O-acetyltransferase (Oac). In this study, we establish the membrane topology of Oac using the dual reporter PhoA-LacZα. We have determined that Oac is an integral membrane protein with 10 transmembrane regions. The hydrophilic N- and C-termini are oriented in the cytoplasm. Functionally important cytoplasmic and periplasmic loops have also been identified. Furthermore, cytoplasmic residues R73 and R75R76 were found to be critical to Oac function.",

keywords = "Critical arginine residues, O-Acetyltransferase, Oac, PhoA-LacZα, Topology",

author = "Farzaana Thanweer and Vikas Tahiliani and Haralambos Korres and Verma, {Naresh K.}",

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Topology and identification of critical residues of the O-acetyltransferase of serotype-converting bacteriophage, SF6, of Shigella flexneri. / Thanweer, Farzaana; Tahiliani, Vikas; Korres, Haralambos et al.
In: Biochemical and Biophysical Research Communications, Vol. 375, No. 4, 31.10.2008, p. 581-585.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Topology and identification of critical residues of the O-acetyltransferase of serotype-converting bacteriophage, SF6, of Shigella flexneri

AU - Thanweer, Farzaana

AU - Tahiliani, Vikas

AU - Korres, Haralambos

AU - Verma, Naresh K.

PY - 2008/10/31

Y1 - 2008/10/31

N2 - The modification of the LPS O-antigen, seen in the diverse serotypes of Shigella flexneri is brought about by the glucosyltransferases (Gtr) and the O-acetyltransferase (Oac). In this study, we establish the membrane topology of Oac using the dual reporter PhoA-LacZα. We have determined that Oac is an integral membrane protein with 10 transmembrane regions. The hydrophilic N- and C-termini are oriented in the cytoplasm. Functionally important cytoplasmic and periplasmic loops have also been identified. Furthermore, cytoplasmic residues R73 and R75R76 were found to be critical to Oac function.

AB - The modification of the LPS O-antigen, seen in the diverse serotypes of Shigella flexneri is brought about by the glucosyltransferases (Gtr) and the O-acetyltransferase (Oac). In this study, we establish the membrane topology of Oac using the dual reporter PhoA-LacZα. We have determined that Oac is an integral membrane protein with 10 transmembrane regions. The hydrophilic N- and C-termini are oriented in the cytoplasm. Functionally important cytoplasmic and periplasmic loops have also been identified. Furthermore, cytoplasmic residues R73 and R75R76 were found to be critical to Oac function.

KW - Critical arginine residues

KW - O-Acetyltransferase

KW - Oac

KW - PhoA-LacZα

KW - Topology

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U2 - 10.1016/j.bbrc.2008.08.069

DO - 10.1016/j.bbrc.2008.08.069

M3 - Article

SN - 0006-291X

VL - 375

SP - 581

EP - 585

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Topology and identification of critical residues of the O-acetyltransferase of serotype-converting bacteriophage, SF6, of Shigella flexneri

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