TY - CONF
T1 - Transcriptomics-Driven Discovery of New Meroterpenoid Rhynchospenes Involved in the Virulence of the Barley Pathogen Rhynchosporium commune
AU - Darma, Reynnaldi
AU - Shang, Zhuo
AU - Bracegirdle, joe
AU - Moggach, Stephen
AU - McDonald, Megan
AU - Piggott, Andrew M.
AU - Solomon, Peter
AU - Chooi, Yit-Heng
PY - 2025
Y1 - 2025
N2 - Rhynchosporiumcommune,thecausalagentofbarley scalddisease, poses amajor threat toglobal barleyproduction. Despite its significant impact, themolecularmechanisms underlyingR.commune’sinfectionprocessremainlargelyunexplored.To addressthis,weanalyzedthedifferentialgeneexpressiondataofR. communeWAI453 cultivatedunder both in planta and in vitro conditions, aiming to identify secondarymetabolitebiosynthetic geneclustersthatarepotentiallyinvolvedinthepathogenicityofR. commune. Our analysis revealed increased expression of a polyketide-terpene gene cluster (the rhy cluster), containing a specificmyeloblastosis(MYB)-typetranscriptionfactorgenerhyM, during in planta growth. Overexpressionof rhyMin an axenic cultureactivatedtheexpressionoftherhycluster,resultingintheproductionofaseriesofnewmeroterpenoidmetabolites,whichwe namedrhynchospenesA−E.TheirstructureswereelucidatedthroughacombinationofspectroscopicmethodsandsinglecrystalXray diffraction analysis. Infiltration of rhynchospenes into barley leaves resulted in strong necrosis, with rhynchospene B demonstratingthehighestphytotoxicityandcausingnecrosisataminimumconcentrationof50ppm.SilencingrhyMinR.commune WAI453confirmed the roleof rhynchospenes as virulence factors inbarleydisease.The resultingmutant showedsignificantly reducedexpressionof therhycluster inplantacomparedtothewild-typestrainanddecreasedvirulenceinseedlingpathogenicity assaysonbarley.Thecharacterizationoftherhyclusterandrhynchospenesprovidedinsightsintotheroleofsecondarymetabolites inR.communevirulenceandbarleyscalddiseasedevelopment.ThestudyalsohighlightsthepotentialuseofMYB-typetranscription factoroverexpressioninuncoveringcrypticSMs involvedinpathogenicityandhostadaptations.
AB - Rhynchosporiumcommune,thecausalagentofbarley scalddisease, poses amajor threat toglobal barleyproduction. Despite its significant impact, themolecularmechanisms underlyingR.commune’sinfectionprocessremainlargelyunexplored.To addressthis,weanalyzedthedifferentialgeneexpressiondataofR. communeWAI453 cultivatedunder both in planta and in vitro conditions, aiming to identify secondarymetabolitebiosynthetic geneclustersthatarepotentiallyinvolvedinthepathogenicityofR. commune. Our analysis revealed increased expression of a polyketide-terpene gene cluster (the rhy cluster), containing a specificmyeloblastosis(MYB)-typetranscriptionfactorgenerhyM, during in planta growth. Overexpressionof rhyMin an axenic cultureactivatedtheexpressionoftherhycluster,resultingintheproductionofaseriesofnewmeroterpenoidmetabolites,whichwe namedrhynchospenesA−E.TheirstructureswereelucidatedthroughacombinationofspectroscopicmethodsandsinglecrystalXray diffraction analysis. Infiltration of rhynchospenes into barley leaves resulted in strong necrosis, with rhynchospene B demonstratingthehighestphytotoxicityandcausingnecrosisataminimumconcentrationof50ppm.SilencingrhyMinR.commune WAI453confirmed the roleof rhynchospenes as virulence factors inbarleydisease.The resultingmutant showedsignificantly reducedexpressionof therhycluster inplantacomparedtothewild-typestrainanddecreasedvirulenceinseedlingpathogenicity assaysonbarley.Thecharacterizationoftherhyclusterandrhynchospenesprovidedinsightsintotheroleofsecondarymetabolites inR.communevirulenceandbarleyscalddiseasedevelopment.ThestudyalsohighlightsthepotentialuseofMYB-typetranscription factoroverexpressioninuncoveringcrypticSMs involvedinpathogenicityandhostadaptations.
U2 - 10.1021/acschembio.4c00731
DO - 10.1021/acschembio.4c00731
M3 - Paper
ER -