@inbook{bde7e92a05e74fd4b1a03176aacec120,
title = "Transfection of plasmodium falciparum",
abstract = "Genetic manipulation of Plasmodium falciparum remains very challenging, mainly due to the parasite genome's high A/T-richness and low transfection efficiency. This chapter includes methods for generating transient and stable transfections by electroporation, allelic replacement with tagged genes, gene deletion, and the analysis of all the above.",
keywords = "Allelic replacement, Electroporation, Knockout, Negative selectable markers, Plasmodium falciparum, Positive selectable markers, Transfection, Vectors",
author = "Melanie Rug and Alexander Maier",
year = "2013",
doi = "10.1007/978-1-62703-026-7_6",
language = "English",
isbn = "9781627030250",
volume = "923",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "75--98",
editor = "{Robert Menard}",
booktitle = "Malaria: Methods and Protocols",
edition = "2nd",
}