Transfection of the human malaria parasite Plasmodium falciparum.

Brendan S. Crabb; Melanie Rug; Tim Wolf Gilberger; Jennifer K. Thompson; Tony Triglia; Alexander G. Maier; Alan F. Cowman

doi:10.1385/1-59259-793-9:263

Transfection of the human malaria parasite Plasmodium falciparum.

Brendan S. Crabb^*, Melanie Rug, Tim Wolf Gilberger, Jennifer K. Thompson, Tony Triglia, Alexander G. Maier, Alan F. Cowman

^*Corresponding author for this work

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163 Citations (Scopus)

Abstract

Methods to transiently and stably transfect blood stages of the human malaria parasite Plasmodium falciparum have been developed and adapted for gene-knockout, allelic replacement, and transgene expression in this organism. These methods are detailed in this chapter, as are approaches used to monitor transfectants during the selection process. The different plasmid vectors that are currently used for gene targeting and transgene expression (including green fluorescent protein expression) are also described.

Original language	English
Pages (from-to)	263-276
Number of pages	14
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	270
DOIs	https://doi.org/10.1385/1-59259-793-9:263
Publication status	Published - 2004
Externally published	Yes

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10.1385/1-59259-793-9:263

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Transfection of the human malaria parasite Plasmodium falciparum.

Abstract

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