Transport of glutathione transferase-fold structured proteins into living cells

Melanie J. Morris; Scott J. Craig; Theresa M. Sutherland; Philip G. Board; Marco G. Casarotto

doi:10.1016/j.bbamem.2008.10.018

Transport of glutathione transferase-fold structured proteins into living cells

Melanie J. Morris, Scott J. Craig, Theresa M. Sutherland, Philip G. Board, Marco G. Casarotto^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Glutathione transferases are a family of enzymes that are traditionally known to contribute to the phase II class of detoxification reactions. However, a novel property of the Schistosoma japonicum glutathione transferase (Sj.GST26) involves its translocation from the external medium into a variety of different cell types. Here we explore the efficiency and mechanism of cell entry for this class of protein. Using flow cytometry and confocal microscopy, we have examined the internalisation of Sj.GST26 into live cells under a variety of conditions designed to shed light on the mode of cellular uptake. Our results show that Sj.GST26 can effectively enter cells through an energy-dependent event involving endocytosis. More specifically, Sj.GST26 was found to colocalise with transferrin within the cell indicating that the endocytosis process involves clathrin-coated pits. A comprehensive study into the cellular internalisation of proteins from other classes within the GST structural superfamily has also been conducted. These experiments suggest that the 'GST-fold' structural motif influences cellular uptake, which presents a novel glimpse into an unknown aspect of GST function.

Original language	English
Pages (from-to)	676-685
Number of pages	10
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1788
Issue number	3
DOIs	https://doi.org/10.1016/j.bbamem.2008.10.018
Publication status	Published - Mar 2009

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title = "Transport of glutathione transferase-fold structured proteins into living cells",

abstract = "Glutathione transferases are a family of enzymes that are traditionally known to contribute to the phase II class of detoxification reactions. However, a novel property of the Schistosoma japonicum glutathione transferase (Sj.GST26) involves its translocation from the external medium into a variety of different cell types. Here we explore the efficiency and mechanism of cell entry for this class of protein. Using flow cytometry and confocal microscopy, we have examined the internalisation of Sj.GST26 into live cells under a variety of conditions designed to shed light on the mode of cellular uptake. Our results show that Sj.GST26 can effectively enter cells through an energy-dependent event involving endocytosis. More specifically, Sj.GST26 was found to colocalise with transferrin within the cell indicating that the endocytosis process involves clathrin-coated pits. A comprehensive study into the cellular internalisation of proteins from other classes within the GST structural superfamily has also been conducted. These experiments suggest that the 'GST-fold' structural motif influences cellular uptake, which presents a novel glimpse into an unknown aspect of GST function.",

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author = "Morris, {Melanie J.} and Craig, {Scott J.} and Sutherland, {Theresa M.} and Board, {Philip G.} and Casarotto, {Marco G.}",

year = "2009",

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doi = "10.1016/j.bbamem.2008.10.018",

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AU - Morris, Melanie J.

AU - Craig, Scott J.

AU - Sutherland, Theresa M.

AU - Board, Philip G.

AU - Casarotto, Marco G.

PY - 2009/3

Y1 - 2009/3

N2 - Glutathione transferases are a family of enzymes that are traditionally known to contribute to the phase II class of detoxification reactions. However, a novel property of the Schistosoma japonicum glutathione transferase (Sj.GST26) involves its translocation from the external medium into a variety of different cell types. Here we explore the efficiency and mechanism of cell entry for this class of protein. Using flow cytometry and confocal microscopy, we have examined the internalisation of Sj.GST26 into live cells under a variety of conditions designed to shed light on the mode of cellular uptake. Our results show that Sj.GST26 can effectively enter cells through an energy-dependent event involving endocytosis. More specifically, Sj.GST26 was found to colocalise with transferrin within the cell indicating that the endocytosis process involves clathrin-coated pits. A comprehensive study into the cellular internalisation of proteins from other classes within the GST structural superfamily has also been conducted. These experiments suggest that the 'GST-fold' structural motif influences cellular uptake, which presents a novel glimpse into an unknown aspect of GST function.

AB - Glutathione transferases are a family of enzymes that are traditionally known to contribute to the phase II class of detoxification reactions. However, a novel property of the Schistosoma japonicum glutathione transferase (Sj.GST26) involves its translocation from the external medium into a variety of different cell types. Here we explore the efficiency and mechanism of cell entry for this class of protein. Using flow cytometry and confocal microscopy, we have examined the internalisation of Sj.GST26 into live cells under a variety of conditions designed to shed light on the mode of cellular uptake. Our results show that Sj.GST26 can effectively enter cells through an energy-dependent event involving endocytosis. More specifically, Sj.GST26 was found to colocalise with transferrin within the cell indicating that the endocytosis process involves clathrin-coated pits. A comprehensive study into the cellular internalisation of proteins from other classes within the GST structural superfamily has also been conducted. These experiments suggest that the 'GST-fold' structural motif influences cellular uptake, which presents a novel glimpse into an unknown aspect of GST function.

KW - Endocytosis

KW - Glutathione transferase

KW - Protein structure

KW - Protein transduction domain

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DO - 10.1016/j.bbamem.2008.10.018

M3 - Article

SN - 0005-2736

VL - 1788

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EP - 685

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

IS - 3

ER -

Transport of glutathione transferase-fold structured proteins into living cells

Abstract

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