Tumor necrosis factor receptor 2 mRNA in rat models of hypertension

Ruby C.Y. Lin, Chris G. Schyvens, Yi Zhang, Judith A. Whitworth, Brian J. Morris*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    In human hypertension (HT) plasma tumor necrosis factor (TNF-α) and soluble TNF receptor 2 fragment (sTNF-R2) are increased, and the TNF-R2 gene (TNFRSF1B) has been implicated. Therefore, we measured Tnfr2 mRNA in kidney, adrenal, heart, and aorta from rats with ACTH-induced, corticosterone-induced, and spontaneous HT (SHR), and tested the effect of blockade of TNF-α by a recombinant TNF-R2 fragment (huTNFR:Fc) on development of HT in the ACTH model. Tnfr2 mRNA was quantified by real-time polymerase chain reaction, as were internal controls, β-actin, and glyceraldehyde-3-phosphate dehydrogenase mRNA. The results showed no differences in tissue Tnfr2 mRNA between HT and control rats. The ACTH-induced HT was not affected by huTNFR:Fc co-administration. The findings thus offer no support for altered Tnfr2 expression in the rat models studied.

    Original languageEnglish
    Pages (from-to)685-688
    Number of pages4
    JournalAmerican Journal of Hypertension
    Volume16
    Issue number8
    DOIs
    Publication statusPublished - 1 Aug 2003

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