TY - JOUR
T1 - Tumor necrosis factor receptor 2 mRNA in rat models of hypertension
AU - Lin, Ruby C.Y.
AU - Schyvens, Chris G.
AU - Zhang, Yi
AU - Whitworth, Judith A.
AU - Morris, Brian J.
PY - 2003/8/1
Y1 - 2003/8/1
N2 - In human hypertension (HT) plasma tumor necrosis factor (TNF-α) and soluble TNF receptor 2 fragment (sTNF-R2) are increased, and the TNF-R2 gene (TNFRSF1B) has been implicated. Therefore, we measured Tnfr2 mRNA in kidney, adrenal, heart, and aorta from rats with ACTH-induced, corticosterone-induced, and spontaneous HT (SHR), and tested the effect of blockade of TNF-α by a recombinant TNF-R2 fragment (huTNFR:Fc) on development of HT in the ACTH model. Tnfr2 mRNA was quantified by real-time polymerase chain reaction, as were internal controls, β-actin, and glyceraldehyde-3-phosphate dehydrogenase mRNA. The results showed no differences in tissue Tnfr2 mRNA between HT and control rats. The ACTH-induced HT was not affected by huTNFR:Fc co-administration. The findings thus offer no support for altered Tnfr2 expression in the rat models studied.
AB - In human hypertension (HT) plasma tumor necrosis factor (TNF-α) and soluble TNF receptor 2 fragment (sTNF-R2) are increased, and the TNF-R2 gene (TNFRSF1B) has been implicated. Therefore, we measured Tnfr2 mRNA in kidney, adrenal, heart, and aorta from rats with ACTH-induced, corticosterone-induced, and spontaneous HT (SHR), and tested the effect of blockade of TNF-α by a recombinant TNF-R2 fragment (huTNFR:Fc) on development of HT in the ACTH model. Tnfr2 mRNA was quantified by real-time polymerase chain reaction, as were internal controls, β-actin, and glyceraldehyde-3-phosphate dehydrogenase mRNA. The results showed no differences in tissue Tnfr2 mRNA between HT and control rats. The ACTH-induced HT was not affected by huTNFR:Fc co-administration. The findings thus offer no support for altered Tnfr2 expression in the rat models studied.
KW - Adrenocorticotropin-induced hypertension
KW - Corticosterone-induced hypertension
KW - Real-time quantitative reverse transcriptase PCR
KW - Spontaneously hypertensive rat
KW - Tumor necrosis factor receptor 2 mRNA
KW - huTNFR:Fc
UR - http://www.scopus.com/inward/record.url?scp=0041866838&partnerID=8YFLogxK
U2 - 10.1016/S0895-7061(03)00916-6
DO - 10.1016/S0895-7061(03)00916-6
M3 - Article
SN - 0895-7061
VL - 16
SP - 685
EP - 688
JO - American Journal of Hypertension
JF - American Journal of Hypertension
IS - 8
ER -