Tunable Carbon-Dot-Based Dual-Emission Fluorescent Nanohybrids for Ratiometric Optical Thermometry in Living Cells

Chuanxi Wang; Huihui Lin; Zhenzhu Xu; Yijun Huang; Mark G. Humphrey; Chi Zhang

doi:10.1021/acsami.5b11317

Tunable Carbon-Dot-Based Dual-Emission Fluorescent Nanohybrids for Ratiometric Optical Thermometry in Living Cells

Chuanxi Wang, Huihui Lin, Zhenzhu Xu, Yijun Huang, Mark G. Humphrey, Chi Zhang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

192 Citations (Scopus)

Abstract

The use of carbon-dot-based dual-emission fluorescent nanohybrids (DEFNs) as versatile nanothermometry devices for spatially resolved temperature measurements in living cells is demonstrated. The carbon dots (CDs) are prepared in the organic phase and display tunable photoluminescence (PL) across a wide visible range by adjusting the excitation wavelengths and extend of N-doping. DEFNs are formed in a straightforward fashion from CDs (emitting blue PL) and gold nanoclusters (AuNCs, emitting red PL). The DEFNs display ideal single-excitation, dual-emission with two well-resolved, intensity-comparable fluorescence peaks, and function in optical thermometry with high reliability and accuracy by exploiting the temperature sensitivity of their fluorescence intensity ratio (blue/red). Furthermore, the DEFNs have been introduced into cells, exhibiting good biocompatibility, and have facilitated physiological temperature measurements in the range of 25-45 °C; the DEFNs can therefore function as 'non-contact' tools for the accurate measurement of temperature and its gradient inside a living cell.

Original language	English
Pages (from-to)	6621-6628
Number of pages	8
Journal	ACS applied materials & interfaces
Volume	8
Issue number	10
DOIs	https://doi.org/10.1021/acsami.5b11317
Publication status	Published - 16 Mar 2016

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title = "Tunable Carbon-Dot-Based Dual-Emission Fluorescent Nanohybrids for Ratiometric Optical Thermometry in Living Cells",

abstract = "The use of carbon-dot-based dual-emission fluorescent nanohybrids (DEFNs) as versatile nanothermometry devices for spatially resolved temperature measurements in living cells is demonstrated. The carbon dots (CDs) are prepared in the organic phase and display tunable photoluminescence (PL) across a wide visible range by adjusting the excitation wavelengths and extend of N-doping. DEFNs are formed in a straightforward fashion from CDs (emitting blue PL) and gold nanoclusters (AuNCs, emitting red PL). The DEFNs display ideal single-excitation, dual-emission with two well-resolved, intensity-comparable fluorescence peaks, and function in optical thermometry with high reliability and accuracy by exploiting the temperature sensitivity of their fluorescence intensity ratio (blue/red). Furthermore, the DEFNs have been introduced into cells, exhibiting good biocompatibility, and have facilitated physiological temperature measurements in the range of 25-45 °C; the DEFNs can therefore function as 'non-contact' tools for the accurate measurement of temperature and its gradient inside a living cell.",

keywords = "carbon dots, dual-emission nanohybrid, living cells, nanothermometry, tunable fluorescence",

author = "Chuanxi Wang and Huihui Lin and Zhenzhu Xu and Yijun Huang and Humphrey, {Mark G.} and Chi Zhang",

note = "Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

year = "2016",

month = mar,

day = "16",

doi = "10.1021/acsami.5b11317",

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T1 - Tunable Carbon-Dot-Based Dual-Emission Fluorescent Nanohybrids for Ratiometric Optical Thermometry in Living Cells

AU - Wang, Chuanxi

AU - Lin, Huihui

AU - Xu, Zhenzhu

AU - Huang, Yijun

AU - Humphrey, Mark G.

AU - Zhang, Chi

PY - 2016/3/16

Y1 - 2016/3/16

N2 - The use of carbon-dot-based dual-emission fluorescent nanohybrids (DEFNs) as versatile nanothermometry devices for spatially resolved temperature measurements in living cells is demonstrated. The carbon dots (CDs) are prepared in the organic phase and display tunable photoluminescence (PL) across a wide visible range by adjusting the excitation wavelengths and extend of N-doping. DEFNs are formed in a straightforward fashion from CDs (emitting blue PL) and gold nanoclusters (AuNCs, emitting red PL). The DEFNs display ideal single-excitation, dual-emission with two well-resolved, intensity-comparable fluorescence peaks, and function in optical thermometry with high reliability and accuracy by exploiting the temperature sensitivity of their fluorescence intensity ratio (blue/red). Furthermore, the DEFNs have been introduced into cells, exhibiting good biocompatibility, and have facilitated physiological temperature measurements in the range of 25-45 °C; the DEFNs can therefore function as 'non-contact' tools for the accurate measurement of temperature and its gradient inside a living cell.

AB - The use of carbon-dot-based dual-emission fluorescent nanohybrids (DEFNs) as versatile nanothermometry devices for spatially resolved temperature measurements in living cells is demonstrated. The carbon dots (CDs) are prepared in the organic phase and display tunable photoluminescence (PL) across a wide visible range by adjusting the excitation wavelengths and extend of N-doping. DEFNs are formed in a straightforward fashion from CDs (emitting blue PL) and gold nanoclusters (AuNCs, emitting red PL). The DEFNs display ideal single-excitation, dual-emission with two well-resolved, intensity-comparable fluorescence peaks, and function in optical thermometry with high reliability and accuracy by exploiting the temperature sensitivity of their fluorescence intensity ratio (blue/red). Furthermore, the DEFNs have been introduced into cells, exhibiting good biocompatibility, and have facilitated physiological temperature measurements in the range of 25-45 °C; the DEFNs can therefore function as 'non-contact' tools for the accurate measurement of temperature and its gradient inside a living cell.

KW - carbon dots

KW - dual-emission nanohybrid

KW - living cells

KW - nanothermometry

KW - tunable fluorescence

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DO - 10.1021/acsami.5b11317

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SN - 1944-8244

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JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

IS - 10

ER -

Tunable Carbon-Dot-Based Dual-Emission Fluorescent Nanohybrids for Ratiometric Optical Thermometry in Living Cells

Abstract

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