Abstract
We have used m situ hybridization and other molecular methods such as PRINS (PRimed IN Situ hybridization) and in situ
PCR in conjunction with transmission electron microscopy and confocal laser scanning microscopy to study the localization of
viroids at the ultrastructural and histological levels. Avocado sunblolch viroid (ASBVd), citrus exocortis viroid (CEVd), and
coconut cadang cadang viroid (CCCVd) were localized using these techniques, and the results of all three localization studies
are diftereot. ASBVd is localized to the chloroplastic thylakoid membranes, CEVd is spread across the entire nucleus, whereas
CCCVd is spread throughout the nucleus, but with a marked concentration within the dense fibrillar peripheral region of the
nucleolus. While the results of these studies provide some further evidence for the taxonomic differentiation between ASBVd and
the potato spindle tuber group of viroids, they also raise important questions as to the involvement of particular RNA polymerases
in the replication of these viroids. Initial results were published in 1994 (Bonfiglioli et al.). In studies initiated recently, the
ultrastructural localization of hop stunt viroid and also viroids in the apple scar skin viroid group are being investigated. (L)
PCR in conjunction with transmission electron microscopy and confocal laser scanning microscopy to study the localization of
viroids at the ultrastructural and histological levels. Avocado sunblolch viroid (ASBVd), citrus exocortis viroid (CEVd), and
coconut cadang cadang viroid (CCCVd) were localized using these techniques, and the results of all three localization studies
are diftereot. ASBVd is localized to the chloroplastic thylakoid membranes, CEVd is spread across the entire nucleus, whereas
CCCVd is spread throughout the nucleus, but with a marked concentration within the dense fibrillar peripheral region of the
nucleolus. While the results of these studies provide some further evidence for the taxonomic differentiation between ASBVd and
the potato spindle tuber group of viroids, they also raise important questions as to the involvement of particular RNA polymerases
in the replication of these viroids. Initial results were published in 1994 (Bonfiglioli et al.). In studies initiated recently, the
ultrastructural localization of hop stunt viroid and also viroids in the apple scar skin viroid group are being investigated. (L)
| Original language | English |
|---|---|
| Journal | Phytoparasitica |
| Volume | 25 |
| Issue number | 3 |
| Publication status | Published - Aug 1997 |
| Externally published | Yes |
| Event | Xth International Congress of Virology - Jerusalem, Israel Duration: 11 Aug 1996 → 16 Aug 1996 |